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Microfluidic bubble JET flow CELL sorter for cell therapy

Objective

'T cell based cancer therapy has been recognized as one of the most promising future cancer therapy methods. In a typical T cell therapy course, relevant T cells are isolated from the patient’s blood, modified against certain cancer markers, and infused back into the patient’s body where the engineered T cells direct the immune system to attack and finally eliminate the malignant tumor cells and tissues.
Recent clinical research demonstrated that purified T cell subsets could greatly improve the therapy efficacy. However, currently available T cell enrichment techniques including fluorescence activated cell sorting and immunomagnetic isolation are either too expensive and risky, or they lack the multi-marker support. Therefore, there is a need for safe, affordable and multi-marker based T cell enrichment methods for cell therapy applications.
In ERC-SCALPEL project, we developed a thermal vapor bubble jet flow technique for high speed fluorescence cell sorting. The bubble jet flow sorter was based on a precisely fabricated metal heater in a silicon chip. A short current pulse applied to this metal heater induced an array of micro vapor bubbles and consequently a jet flow for high speed aerosol-free cell sorting at 4,000 cells/sec. The bubble jet sorter has shown promise to serve the cell therapy demand: high throughput, high flexibility, guaranteed biosafety and affordability.
The JetCell project aims at carrying out a feasibility study of bubble jet sorting technology for the enrichment of T cells as the first step of a typical cell manufacturing process for cancer cell therapy. With a prototype setup, the bubble jet sorter will be assessed in typical pre-clinical cell manufacturing models, from human cell lines to primary blood cells. Meanwhile, the cell therapy manufacturing market will be further analyzed and an initial business development strategy will be investigated.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.

Funding Scheme

Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.

ERC-POC - Proof of Concept Grant

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Call for proposal

Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.

(opens in new window) ERC-2016-PoC

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Host institution

INTERUNIVERSITAIR MICRO-ELECTRONICA CENTRUM
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 149 379,00
Address
KAPELDREEF 75
3001 Leuven
Belgium

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Region
Vlaams Gewest Prov. Vlaams-Brabant Arr. Leuven
Activity type
Research Organisations
Links
Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 149 379,00

Beneficiaries (1)

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