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Developing Multipurpose Nicotiana Crops for Molecular Farming using New Plant Breeding Techniques

Deliverables

Development of Apollo web based genome browser for PacBio N. benthamiana genome assembly

Development of Apollo web based genome browser for PacBio N. benthamiana genome assembly

Communication Plan and Gender Action Plan

Communication Plan and Gender Action Plan

Initial report on Stakeholder information of NPBTs and Molecular Farming

Initial report on Stakeholder information of NPBTs and Molecular Farming

Collection of editing enzymes with compared efficiencies and predictive model

Collection of editing enzymes with compared efficiencies and predictive model

Initial Report on Regulation and Biosafety updates

Initial Report on Regulation and Biosafety updates

Dissemination Plan

Dissemination Plan

PacBio sequencing and assembly of N. benthaminana genome

PacBio sequencing and assembly of N. benthaminana genome

Searching for OpenAIRE data...

Publications

Engineering CRISPR/ Lb Cas12a for highly efficient, temperature‐tolerant plant gene editing

Author(s): Patrick Schindele, Holger Puchta
Published in: Plant Biotechnology Journal, Issue 18/5, 2020, Page(s) 1118-1120, ISSN 1467-7644
DOI: 10.1111/pbi.13275

Enhancing in planta gene targeting efficiencies in Arabidopsis using temperature‐tolerant CRISPR/ Lb Cas12a

Author(s): Laura Merker, Patrick Schindele, Teng‐Kuei Huang, Felix Wolter, Holger Puchta
Published in: Plant Biotechnology Journal, Issue 18/12, 2020, Page(s) 2382-2384, ISSN 1467-7644
DOI: 10.1111/pbi.13426

Plant molecular farming for the production of valuable proteins – Critical evaluation of achievements and future challenges

Author(s): Stefan Schillberg, Ricarda Finnern
Published in: Journal of Plant Physiology, Issue 258-259, 2021, Page(s) 153359, ISSN 0176-1617
DOI: 10.1016/j.jplph.2020.153359

New Hope for a “Cursed” Crop? Understanding Stakeholder Attitudes to Plant Molecular Farming With Modified Tobacco in Europe

Author(s): Jonathan Menary, Mario Amato, Andrés Cid Sanchez, Matthew Hobbs, Agata Pacho, Sebastian S. Fuller
Published in: Frontiers in Plant Science, Issue 11, 2020, ISSN 1664-462X
DOI: 10.3389/fpls.2020.00791

Expanding the genome-targeting scope and the site selectivity of high-precision base editors

Author(s): Junjie Tan, Fei Zhang, Daniel Karcher, Ralph Bock
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
DOI: 10.1038/s41467-020-14465-z

Shotguns vs Lasers: Identifying barriers and facilitators to scaling-up plant molecular farming for high-value health products

Author(s): Jonathan Menary, Matthew Hobbs, Sara Mesquita de Albuquerque, Agata Pacho, Pascal M. W. Drake, Alison Prendiville, Julian K-C. Ma, Sebastian S. Fuller
Published in: PLOS ONE, Issue 15/3, 2020, Page(s) e0229952, ISSN 1932-6203
DOI: 10.1371/journal.pone.0229952

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Author(s): Borja Diego-Martin, Beatriz González, Marta Vazquez-Vilar, Sara Selma, Rubén Mateos-Fernández, Silvia Gianoglio, Asun Fernández-del-Carmen, Diego Orzáez
Published in: Frontiers in Plant Science, Issue 11, 2020, ISSN 1664-462X
DOI: 10.3389/fpls.2020.612781

ABCC transporters mediate the vacuolar accumulation of crocins in saffron stigmas

Author(s): Olivia Costantina Demurtas, Rita Francisco, Gianfranco Diretto, Paola Ferrante, Sarah Frusciante, Marco Pietrella, Giuseppe Aprea, Lorenzo Borghi, Mistianne Feeney, Lorenzo Frigerio, Adriana Coricello, Giosuè Costa, Stefano Alcaro, Enrico Martinoia, Giovanni Giuliano
Published in: The Plant Cell, 2019, Page(s) tpc.00193.2019, ISSN 1040-4651
DOI: 10.1105/tpc.19.00193

Efficient gene targeting in Nicotiana tabacum using CRISPR/SaCas9 and temperature tolerant LbCas12a

Author(s): Teng‐Kuei Huang, Brittney Armstrong, Patrick Schindele, Holger Puchta
Published in: Plant Biotechnology Journal, 2021, ISSN 1467-7644
DOI: 10.1111/pbi.13546

Assessment of Cas12a‐mediated gene editing efficiency in plants

Author(s): Joan Miquel Bernabé‐Orts, Iván Casas‐Rodrigo, Eugenio G. Minguet, Viola Landolfi, Victor Garcia‐Carpintero, Silvia Gianoglio, Marta Vázquez‐Vilar, Antonio Granell, Diego Orzaez
Published in: Plant Biotechnology Journal, 2019, ISSN 1467-7644
DOI: 10.1111/pbi.13113

Novel biotechnological approaches to produce biological compounds: challenges and opportunities for science communication

Author(s): Lei Pei, Markus Schmidt
Published in: Current Opinion in Biotechnology, Issue 56, 2019, Page(s) 43-47, ISSN 0958-1669
DOI: 10.1016/j.copbio.2018.08.012

Activity‐based proteomics reveals nine target proteases for the recombinant protein‐stabilizing inhibitor Sl CYS 8 in Nicotiana benthamiana

Author(s): Philippe V. Jutras, Friederike Grosse‐Holz, Farnusch Kaschani, Markus Kaiser, Dominique Michaud, Renier A.L. Hoorn
Published in: Plant Biotechnology Journal, Issue 17, 2019, Page(s) 1670–1678, ISSN 1467-7644
DOI: 10.1111/pbi.13092

Engineering of high-precision base editors for site-specific single nucleotide replacement

Author(s): Junjie Tan; Fei Zhang; Daniel Karcher; Ralph Bock
Published in: Nature Communications, Issue 1, 2019, ISSN 2041-1723
DOI: 10.5281/zenodo.2586572

High-level expression of the HIV entry inhibitor griffithsin from the plastid genome and retention of biological activity in dried tobacco leaves

Author(s): Matthijs Hoelscher, Nadine Tiller, Audrey Y.-H. Teh, Guo-Zhang Wu, Julian K-C. Ma, Ralph Bock
Published in: Plant Molecular Biology, Issue 97/4-5, 2018, Page(s) 357-370, ISSN 0167-4412
DOI: 10.1007/s11103-018-0744-7

Construction of a fusion enzyme for astaxanthin formation and its characterisation in microbial and plant hosts: A new tool for engineering ketocarotenoids

Author(s): Marilise Nogueira, Eugenia M.A. Enfissi, Ralf Welsch, Peter Beyer, Matias D. Zurbriggen, Paul D. Fraser
Published in: Metabolic Engineering, Issue 52, 2019, Page(s) 243-252, ISSN 1096-7176
DOI: 10.1016/j.ymben.2018.12.006

Critical Analysis of the Commercial Potential of Plants for the Production of Recombinant Proteins

Author(s): Stefan Schillberg, Nicole Raven, Holger Spiegel, Stefan Rasche, Matthias Buntru
Published in: Frontiers in Plant Science, Issue 10, 2019, ISSN 1664-462X
DOI: 10.3389/fpls.2019.00720

Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds

Author(s): Norma Aliaga-Franco, Cunjin Zhang, Silvia Presa, Anjil K. Srivastava, Antonio Granell, David Alabadí, Ari Sadanandom, Miguel A. Blázquez, Eugenio G. Minguet
Published in: Frontiers in Plant Science, Issue 10, 2019, ISSN 1664-462X
DOI: 10.3389/fpls.2019.01150