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Quantitative detection of bacterial endotoxin by novel nanotechnological approaches

Quantitative detection of bacterial endotoxin by novel nanotechnological approaches

Objective

ENDONANO (Quantitative detection of bacterial endotoxin by novel nanotechnological approaches) addresses a key regulatory and safety issue, i.e., the unbiased quantitative detection of bacterial endotoxin in products for medical use and in drug development and toxicological studies. The currently adopted LAL assays are reliable only in limited conditions and prone to interference at several levels, and therefore cannot be applied to all products and substances. ENDONANO will exploit new concepts, based on the capacity of metal nanoparticles to adsorb endotoxin, and new detection methods, based on molecular beacons, for developing novel assays to quantitatively detect endotoxin in complex matrices and in a wide range of conditions. The scientific and technological goals of ENDONANO include: 1. Investigating the capacity of endotoxin to specifically inducing inflammatory reactions in human primary blood cells; 2. Developing new methods based on endotoxin capture by metal nanoparticles in complex matrices (biological fluids, emulsions, gels, etc.); 3. Designing and implementing signal generation and detection methods for the quantitative endotoxin measurement; 4. Planning assay prototypes to be developed and validated for commercial purposes. ENDONANO will train 4 PhD students in an overarching training programme that will include training-by-research, courses of technical, scientific, and transferrable skills, active participation to public scientific events, and intense intersectoral networking. The ENDONANO consortium encompasses three academic institutions with strong expertise in inflammation, advanced biosensing, and top expertise in nanotechnology and use of nanoparticles for modulating bacterial functions, two SMEs expert in development and commercialisation of diagnostic detection assays and one biotech company specialised in (magnetic) microbead technology, and two Participant Organisations (SMEs). All have proven experience in higher education and training,
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Coordinator

CONSIGLIO NAZIONALE DELLE RICERCHE

Address

Piazzale Aldo Moro 7
00185 Roma

Italy

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 261 499,68

Participants (5)

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PARIS-LODRON-UNIVERSITAT SALZBURG

Austria

EU Contribution

€ 132 103,62

FUNDACIO INSTITUT CATALA DE NANOCIENCIA I NANOTECNOLOGIA

Spain

EU Contribution

€ 125 452,44

DIAGNOSTICUM GYARTO ES FORGALMAZO ZARTKORUEN MUKODO RESZVENYTARSASAG

Hungary

EU Contribution

€ 114 857,64

MILTENYI BIOTEC GMBH

Germany

EU Contribution

€ 126 394,20

BLUSENSE DIAGNOSTICS APS

Denmark

EU Contribution

€ 297 522

Partners (2)

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APPLIED NANOPARTICLES SL

ALTA RICERCA E SVILUPPO IN BIOTECNOLOGIE SRLU

Project information

Grant agreement ID: 812661

Status

Ongoing project

  • Start date

    1 January 2019

  • End date

    31 December 2022

Funded under:

H2020-EU.1.3.1.

  • Overall budget:

    € 1 057 829,58

  • EU contribution

    € 1 057 829,58

Coordinated by:

CONSIGLIO NAZIONALE DELLE RICERCHE

Italy