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Quantitative detection of bacterial endotoxin by novel nanotechnological approaches

Objective

ENDONANO (Quantitative detection of bacterial endotoxin by novel nanotechnological approaches) addresses a key regulatory and safety issue, i.e. the unbiased quantitative detection of bacterial endotoxin in products for medical use and in drug development and toxicological studies. The currently adopted LAL assays are reliable only in limited conditions and prone to interference at several levels, and therefore cannot be applied to all products and substances. ENDONANO will exploit new concepts, based on the capacity of metal nanoparticles to adsorb endotoxin, and new detection methods, based on molecular beacons, for developing novel assays to quantitatively detect endotoxin in complex matrices and in a wide range of conditions. The scientific and technological goals of ENDONANO include: 1. Investigating the capacity of endotoxin to specifically inducing inflammatory reactions in human primary blood cells; 2. Developing new methods based on endotoxin capture by metal nanoparticles in complex matrices (biological fluids, emulsions, gels, etc.); 3. Designing and implementing signal generation and detection methods for the quantitative endotoxin measurement; 4. Planning assay prototypes to be developed and validated for commercial purposes. ENDONANO will train 4 PhD students in an overarching training programme that will include training-by-research, courses of technical, scientific, and transferrable skills, active participation to public scientific events, and intense intersectoral networking. The ENDONANO consortium encompasses three academic institutions with strong expertise in inflammation, advanced biosensing, and top expertise in nanotechnology and use of nanoparticles for modulating bacterial functions, two SMEs expert in development and commercialisation of diagnostic detection assays and one biotech company specialised in (magnetic) microbead technology, and two Participant Organisations (SMEs). All have proven experience in higher education and training,

Call for proposal

H2020-MSCA-ITN-2018
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Coordinator

CONSIGLIO NAZIONALE DELLE RICERCHE
Address
Piazzale Aldo Moro 7
00185 Roma
Italy
Activity type
Research Organisations
EU contribution
€ 261 499,68

Participants (6)

PARIS-LODRON-UNIVERSITAT SALZBURG
Austria
EU contribution
€ 132 103,62
Address
Kapitelgasse 4-6
5020 Salzburg
Activity type
Higher or Secondary Education Establishments
FUNDACIO INSTITUT CATALA DE NANOCIENCIA I NANOTECNOLOGIA
Spain
EU contribution
€ 90 604,54
Address
Campus De La Uab Edifici Q Icn2
08193 Bellaterra (Barcelona)
Activity type
Research Organisations
DIAGNOSTICUM GYARTO ES FORGALMAZO ZARTKORUEN MUKODO RESZVENYTARSASAG
Hungary
EU contribution
€ 114 857,64
Address
Attila U 126
1047 Budapest
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
MILTENYI BIOTEC GMBH

Participation ended

Germany
EU contribution
€ 0
Address
Friedrich Ebert Strasse 68
51429 Bergish Gladbach
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
BLUSENSE DIAGNOSTICS APS
Denmark
EU contribution
€ 148 761
Address
Fruebjergvej 3
2100 Kobenhavn
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
MILTENYI BIOTEC BV & CO KG
Germany
EU contribution
€ 252 788,40
Address
Friedrich Ebert Strasse 68
51429 Bergisch Gladbach
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)

Partners (2)

APPLIED NANOPARTICLES SL
Spain
Address
Calle Alaba 88
08018 Barcelona
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
ALTA RICERCA E SVILUPPO IN BIOTECNOLOGIE SRLU
Italy
Address
Via Fiorentina 151
53100 Siena
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)