Objective
Since the prophetic vision of the German scientist Emil du Bois-Reymond in 1877 that put forward the hypothesis of the chemical nature of synaptic transmission, a large number of biochemical, genetic and neuro-physiological studies have revealed all the components that are involved in synaptic vesicle (SV) exocytosis. This complex action-potential-triggered process, in which the entry of calcium ions (Ca2+) into the presynaptic compartment induces a milliseconds-fast fusion of neurotransmitter-containing SVs with the presynaptic plasma membrane (PM), requires a number of so-called presynaptic proteins playing different roles. To date, despite all the knowledge acquired about presynaptic proteins, the question how they carry out this fast process remains unanswered. Recently, it was hypothesized that the presynaptic proteins are organized in specific supramolecular arrangements and do cooperate to achieve this remarkable feat. However, the study of presynaptic protein arrangement and functioning at nanometer isotropic scale is challenging, and decades of research were unable to resolve this phenomenon. In this project, I plan to study the three-dimensional supramolecular structures of the presynaptic proteins during SV exocytosis at nanometer isotropic resolution, and its structural dynamics on the nanometer length scale with microsecond temporal resolution. For this purpose, I will combine the super-resolution technique Single-Molecule Metal-Induced Energy Transfer (smMIET), developed by the host group, with the single-molecule localization microscopy (SMLM) methods of DNA-PAINT and MINFLUX. The overall importance of this proposal in understanding the Ca2+-triggered SV fusion is that it is essential to understand how synapses and ultimately the brain work, and all derived knowledge and applications that comes with it.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- natural sciences physical sciences optics microscopy super resolution microscopy
- natural sciences biological sciences biochemistry biomolecules proteins
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2021-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
37073 Gottingen
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.