Project description
Towards improved enzyme-electrode systems
Reduction-oxidation (redox) enzymes facilitate chemical reactions involving the transfer of electrons between molecules. These enzymes are essential in various biological processes, including cellular respiration, photosynthesis, and the metabolism of various compounds. They have also been explored in applications that involve energy conversion, biosensing, and electrosynthesis. However, their limited stability and the challenge of aligning them with electrode surfaces hinder their widespread use. Funded by the Marie Skłodowska-Curie Actions programme, the ReversE project aims to develop a reversible immobilisation method to attach enzymes to electrodes with precise orientation. This approach will enable the controlled release of enzymes upon stimulation, allowing for their regeneration and sustainable deployment in enzyme-electrode systems.
Objective
Redox enzymes catalyze numerous biological processes that are also relevant for a variety of applications, including energy conversion, biosensing, and electrosynthesis of complex molecules. The intrinsic lability of enzymes that gradually lose catalytic activity over time, however, restricts their operational half-life for practical large-scale application of this sustainable and incredibly energy-saving system. Moreover, enzymes are nanometer-sized macromolecules, that must be properly aligned with the electrode surface for efficient electron transfer and substrate access to the active site regardless of the electron transfer mechanism, direct or mediated, which typically necessitates laborious procedures. The goal of this Marie Skłodowska-Curie Postdoctoral Fellowship project is to develop a reversible immobilization approach that can attach enzymes on an electrode surface in a defined orientation and permit their controlled release on demand upon stimulation (ReversE). This will be accomplished by capitalizing on a gold (Au)-binding peptide fused to [FeFe]-hydrogenase as the model enzyme to have the dual functionality of orienting enzymes on the Au electrode surface in a precise manner to support efficient electron transfer and substrate access to the active site, as well as allowing the bound Au-binding peptide fused enzymes to be released by electrochemical or chemical stimuli and replaced with fresh enzymes when the catalytic activity is reduced to a predetermined value. This will open up the possibility for enzyme regeneration which will be groundbreaking in the context of sustainable deployment of enzyme‒electrode systems.
Fields of science (EuroSciVoc)
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CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2022-PF-01
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80333 Muenchen
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.