Objective
The proposed research aims to understand biophysical embryo-uterine interactions during peri-implantation to identify factors that guide processes such as embryo orientation, egg cylinder morphogenesis, and embryonic axis formation. Recent studies on implantation have gone beyond the traditional biochemical approach to reveal that mechanical interactions at the embryo-uterine interface are abundant and indispensable for proper implantation and development. Due to difficulties in recreating embryo implantation ex-vivo setups and lack of in-utero accessibility, the physical forces and mechano-chemical feedbacks involved in embryo implantation, however, still remain unknown.
In this proposal, I will study how mechanical interactions at the embryo-uterine interface during implantation affect embryo development by directly measuring physical force at the interface, identifying the role of major mechanotransducer pathways, and making in-utero observations. In Objective 1, I will build an experimental setup with 2D polyacrylamide (PAA) gels of varying mechanical properties to recapitulate the characteristics of the uterine surface in ex-vivo setups and observe how embryo development depends on the mechanical properties of the substrate. In Objective 2, I will combine traction force microscopy (TFM) and live imaging to quantify how physical forces affect molecular and cellular events during the mouse implantation process. In Objective 3, I will identify how the mechanical forces are transduced into biochemical cues through live-imaging and genetic/pharmaceutical perturbations. Finally, I will investigate the implantation process live in-utero using multiphoton intravital imaging to validate my findings from ex-vivo studies. I hypothesize that mechanical cues will be spatiotemporally linked with biochemical signaling to significantly affect embryo development.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences optics microscopy
- medical and health sciences clinical medicine embryology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2023-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
1011 JV AMSTERDAM
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.