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De novo formation of the generative plant meristem in 4D

Project description

Understanding meristem evolution and formation in land plants

Meristem formation is essential for plant growth, but the process of how stable patterns are established from a single cell remains unclear. Marchantia polymorpha is used to study meristem formation in real time because its single-celled spore undergoes specific cell division patterns to produce a highly organised meristem. Supported by the Marie Skłodowska-Curie Actions programme, the DeNovoMeristem project aims to observe real-time spatial and temporal changes in gene expression during meristem formation and to identify the molecular interactions driving these changes. This project will shed light on how gene expression patterns and feedback loops can form de novo, uncover critical innovations in meristem evolution, and clarify the fundamental requirements for meristem formation in land plants.

Objective

Meristem formation is essential for continuous and organised growth in all land plants. The meristem is a multicellular generative centre that produces the plant body and is maintained by spatially restricted gene expression domains throughout a plant’s lifespan. However, little is known about how these stable patterns are established de novo from a single cell. This is because embryonic meristem formation in flowering plants is enclosed within the seed and fruit and therefore live imaging, chemical treatments and gene interaction studies are technically difficult. The bryophyte Marchantia polymorpha has emerged as a powerful model to investigate meristem formation in real time. The single-celled Marchantia haploid spore is exposed and undergoes defined patterns of cell division to ultimately produce a highly organised meristem. In the proposed project, spatial and temporal gene expression changes will be observed live during meristem formation, and the molecular interactions driving these changes will be identified. As well as revealing how gene expression patterns and feedback loops can form de novo, this work will reveal key innovations during meristem evolution and identify the fundamental requirements for meristem formation in land plants.

Fields of science (EuroSciVoc)

Keywords

Programme(s)

Topic(s)

Funding Scheme

HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

(opens in new window) HORIZON-MSCA-2023-PF-01

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Coordinator

GREGOR MENDEL INSTITUT FUR MOLEKULARE PFLANZENBIOLOGIE GMBH
Net EU contribution
€ 183 600,96
Address
DR BOHR GASSE 3
1030 Wien
Austria
Region
Ostösterreich Wien Wien
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
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Total cost
No data