Project description
Advancing super-resolution microscopy
Being able to observe how biological processes take place at the molecular level, is central to understanding life. Super-resolution microscopy helps researchers visualise molecular structures with nanometre precision. However, current methods fail to combine imaging speed and spatial accuracy. With the support of the Marie Skłodowska-Curie Actions programme, the ES-FLUX project aims to address this limitation by introducing innovative sensors capable of recording light changes with high precision. This approach captures both rich spatial and temporal information simultaneously, improving resolution without sacrificing throughput. The project will advance both computational analysis and instrumentation, hoping to expand the power of high-resolution biological microscopy.
Objective
Non-invasive, high-resolution imaging of biological systems often requires optical microscopy. Since overcoming the diffraction barrier, optical nanoscopy, or super-resolution microscopy, with up to ~1-10 nm resolution can be used for imaging immobilized or live biological samples. The final frontier of optical nanoscopy is high-throughput, true molecular imaging (~1 nm precision) in dynamic, high-mobility systems. Currently, the most promising nanoscopy methods employ modulation-enhanced localization microscopy. However, these are compromised either in throughput or in spatiotemporal precision. This trade-off is due to a sensor choice focused on rich temporal (realizing high localization precision) or rich spatial (realizing high throughput) information. A novel sensor architecture, event-based sensors, merges these choices by measuring changes in photon flux on a megapixel-sized chip with asynchronously recorded microsecond-precise timestamped events. With ES-FLUX, I will combine patterned sinusoidal illumination patterns with event-based single-molecule localization microscopy. This will break the stalemate between high throughput and high precision in modulation-enhanced localization microscopy. The spatiotemporal single-molecule event patterns will be analysed separately in the temporal and in the spatial domains, and as such ES-FLUX will improve the resolution compared to traditional single-molecule localization microscopy. It paves the way for molecular precision whilst allowing the high experimental throughput associated with full-frame camera acquisition. Further improvements in the computational analysis and optomechanical setup expands ES-FLUX towards single-particle tracking and spectral multiplexing. In conclusion, ES-FLUX merges modulation-enhanced single-molecule localization microscopy with event-based sensor acquisition and as such expands the boundaries of optical nanoscopy.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering sensors optical sensors
- natural sciences physical sciences optics microscopy super resolution microscopy
- natural sciences physical sciences theoretical physics particle physics photons
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
MAIN PROGRAMME
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2024-PF-01
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
2628 CN Delft
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.