Objectif The primary objective is to produce basic knowledge on the mechanisms by which a variety of signals control growth and cell differentiation. The secondary objective will be to develop tools at cellular and molecular levels to render morphogenetic events accessible to scientific analysis. Many plant species,including economically important crops,can not be regenerated from single cells in-vitro. We propose to investigate the mechanism controlling the induction of cell proliferation in-vitro. We Will use Brassica napus microspores as a model system since-freshly isolated-microspores can be redirected to an embryo formation pathway at frequencies of up to 70 percent. 32 C heat treatment of an 8 hour duration is sufficient to initiate and sustain the microspore embryogenic process. The B.napus microspore culture system has a number of other characteristics-well suited for such studies. The specific areas to be investigated:genes regulating induction of microspore embryogenesis,regulation of cytoskeletal and other cellular components during the induction phase and inheritance of the induction specific character(s) in a plant population. We will also compare B.napus microspores With Hordeum vulgare(barley) microspores to identify common molecular features-of microspore embryogenesis induction in dicots and monocot. The project proposes to significantly improve our understanding of the inductive phase of microspore embryogenesis in particular and cell proliferation in general. The results Will help us to understand Why microspores of some economically important plant species can not be induced to undergo embryogenic development in-vitro and open the possibility to manipulate and transfer genes regulating induction of microspore(cell) proliferation to tissue culture non-responsive plant species. Champ scientifique natural sciencesbiological sciencesdevelopmental biologyagricultural sciencesagriculture, forestry, and fisheriesagriculturemedical and health sciencesclinical medicineembryology Programme(s) FP2-BRIDGE - Specific research and technological development programme (EEC) in the field of biotechnology (BRIDGE), 1990-1994 Thème(s) Data not available Appel à propositions Data not available Régime de financement CSC - Cost-sharing contracts Coordinateur MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. Contribution de l’UE Aucune donnée Adresse Carl-von-Linné-Weg 10 50829 KOELN Allemagne Voir sur la carte Coût total Aucune donnée Participants (4) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS Espagne Contribution de l’UE Aucune donnée Adresse Calle Serrano 117 MADRID Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée JOHN INNES CENTRE Royaume-Uni Contribution de l’UE Aucune donnée Adresse Norwich Research Park, Colney NORWICH Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Nickerson Biocem Ltd Royaume-Uni Contribution de l’UE Aucune donnée Adresse Cambridge Science Park Milton Road CB4 4GZ Cambridge Voir sur la carte Coût total Aucune donnée WAGENINGEN UNIVERSITY Pays-Bas Contribution de l’UE Aucune donnée Adresse 4,Arboretumlaan 4 6703 BD WAGENINGEN Voir sur la carte Coût total Aucune donnée