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Glycoproteins with homogeneous glycosylation to define unexplored lectin binding properties

Project description

Decoding lectin specificity for cancer glycobiology

Aberrant glycosylation – the attachment of sugars to proteins and lipids – is a hallmark of cancer. Lectins are carbohydrate-binding proteins found in most living organisms and they have proved to be invaluable cancer research tools. With the support of the Marie Skłodowska-Curie Actions programme, the GlycoScope project aims to shed light on lectin’s binding to unexplored glycosylation patterns in cancer. It will combine atomic-level molecular insights from nuclear magnetic resonance spectroscopy with widely used functional lectin assays. Using chemoenzymatic methods, GlycoScope will synthesise pure glycoproteins with homogeneous, naturally occurring sugar modifications, thereby eliminating the confounding heterogeneity found in natural systems. Outcomes will advance the important field of cancer glycobiology.

Objective

GlycoScope aims to provide key answers for the understanding of lectin binding to complex and previously unexplored glycosylation patterns related to cancer. Main aim is to integrate atomic-level insights into molecular recognition, obtained through Nuclear Magnetic Resonance (NMR) spectroscopy, with widely used lectin-based assays for glycosylation analysis. This combined strategy will enable a precise chemical definition of lectin specificities and refine one of the most commonly applied tools in glycosciences. To achieve this, and as a major challenge, pure glycoproteins presenting homogeneous glycoepitopes will be generated through chemoenzymatic synthesis, ensuring natural glycan presentation and providing the optimal platform for lectin-based assays, while overcoming the critical drawback of heterogeneity that characterizes natural glycosylation. Polylactosamine (PLN) modification through I-branching and terminal sialylation will be interrogated for their binding to PLN- binding lectins, including plant lectins commonly used to characterize glycosylation and galectins, immune lectins involved in health and disease.
Complementary, GlycoScope offers Dr. Soriano a unique opportunity to broaden his training beyond his current focus on natural products and synthetic chemistry. It will provide him with interdisciplinary expertise in Molecular Recognition, Biological Chemistry and Glycobiology, thereby greatly enhancing his future employability and broadening his scientific perspective. Alongside the acquisition of specific technical skills—including NMR-based molecular recognition, chemoenzymatic chemistry, and molecular biology and protein biochemistry—Dr. Soriano will also benefit from the outstanding scientific environment and advanced training opportunities available at CIC bioGUNE. At the same time, the host-group will highly benefit from Dr. Sorianos´ expertise in chemical synthesis and purification procedures, key for the development of the project.

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HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships

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Call for proposal

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(opens in new window) HORIZON-MSCA-2025-PF

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Coordinator

ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOCIENCIAS
Net EU contribution

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€ 194 074,56
Address
PARQUE TECNOLOGICO EDIFICIO 801 A
48160 DERIO VIZCAYA
Spain

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Region
Comunidad de Madrid Comunidad de Madrid Madrid
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Research Organisations
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Total cost

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