Project description
Axonal membrane-associated skeleton changes in axonal branching
Neuronal morphology is highly plastic, changing during development, ageing, memory formation and neurodegenerative disorders. Axonal branching – when a single axon sprouts new branches to connect with multiple target cells – is one of these changes. The mechanisms of associated dynamic changes in the axonal membrane-associated skeleton (MPS), an actin-spectrin scaffold, are not known. With the support of the Marie Skłodowska-Curie Actions programme, the PAPS project aims to investigate the hypothesis that absence of MPS at branch initiation in immature neurons or MPS disruption in mature ones is required. Using super-resolution microscopy, live-cell imaging, tagged probes and optogenetic perturbations, it will track and manipulate branching, with implications for neurodevelopmental and neurodegenerative disorders.
Objective
The morphology of neurons is critical for circuit formation in the nervous system, with neuronal arborization requiring precise cytoskeletal and membrane remodeling. The axonal membrane-associated skeleton (MPS) is a scaffold made of actin and spectrin that plays a key role in maintaining axonal integrity. While some of its functions have been characterized, its dynamics during axonal branching remain unknown. The established role of actin, microtubules and mitochondria in regulating branching motivate us to investigate their interplay with the MPS in the context of branch initiation. We hypothesize that branching along immature axons occurs in segments devoid of MPS, and that branch initiation along mature axons requires MPS disruption. Using super-resolution microscopy, live-cell imaging, endogenously-tagged probes and optogenetic perturbations, we will track MPS dynamics, induce branching, and manipulate scaffolds to understand this phenomenon. This will provide insights into how MPS dynamics coordinate axonal plasticity, with implications for neurodevelopmental and neurodegenerative disorders.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project's classification has been human-validated.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
This project's classification has been human-validated.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA)
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Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European Fellowships
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Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) HORIZON-MSCA-2025-PF
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13284 Marseille
France
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