Objective A new family of cognate glycoproteins with chitinase-sensitive carbohydrate moiety, chitinoproteins (ChiP) has been detected in Drosophila melanogaster and other insects. ChiP localisation in a variety of tissues of D. melanogaster was shown to be developmentally regulated, thus providing an opportunity to study the regulation of tissue specific ChiP gene expression. ChiP proved to be secreted both by cultured cells and in vivo, not only by epidermal and blood cells but also by follicle cells into the oöcyte. This suggests that ChiP have multiple functions in insect morphogenesis, including participation in cuticle and extracellular matrix formation, as well as in oögenesis. The following biochemical and genetic studies will therefore be carried out: analysis of the carbohydrate structure of the glycosylation mechanism, including assignment to a particular cellular compartment of different steps of biosynthesis; and microsequencing of ChiP polypeptide to enable cloning of the gene by means of screening of a cDNA library with oligonucleotide probes or PCR-mediated approach. Chromosomal localisation of the gene and isolation of mutants may be an approach to elucidate ChiP functions. Programme(s) IC-INTAS - International Association for the promotion of cooperation with scientists from the independent states of the former Soviet Union (INTAS), 1993- Topic(s) 42 - Physical Chemical Biology Call for proposal Data not available Funding Scheme Data not available Coordinator Biozentrum Universität Basel EU contribution No data Address Klingelbergstraße 70 4056 Basel Switzerland See on map Total cost No data Participants (2) Sort alphabetically Sort by EU Contribution Expand all Collapse all Russian Academy of Sciences Russia EU contribution No data Address 123182 Moscow See on map Total cost No data THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD United Kingdom EU contribution No data Address SOUTH PARKS ROAD OX1 3QU OXFORD See on map Total cost No data
