Objective
The level at which signal-transduction is integrated to give Long-term-potentiation (LTP) is unclear, however, recent evidence implicates the cytoskeleton as important. The characteristic ATP-, calcium-dependent rundown of the NMDA-receptor (which is centrally involved in LTP) is reversed by treatments thought to specifically protect the filamentous state of the cytoskeletal protein actin (F-actin) from depolymerization into its monomeric components (G-actin). Since F-actin depolymerization is regulated by calcium-dependent proteases it is thought that the receptor rundown could be through NMDA-receptor activated cytosolic calcium increases. Currently, there are no studies demonstrating a direct effect of NMDA-receptor stimulation on the state of F-actin. This project will directly determine whether such a link exists, and whether treatments thought to alter the polymerisation state of actin are effective. We will use cultured neurones of the rat hippocampus, and the site-selective probes: phalloidin for F-(polymerized), and DNAaseI for G-(depolymerized) actin. Fluorescent conjugates of these probes will allow visualisation of F-, and G-actin simultaneously in Axed, and living neuronal cells. The distribution and intensity of staining will be monitored in single cells using confocal microscopy after systematic treatments.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences inorganic chemistry alkaline earth metals
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics microscopy confocal microscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
75674 Paris
France
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.