Objective
The c-mos proto-oncogene product is a key element in the cascade of events leading to meiotic maturation of vertebrate oocytes. The expression of c-mos mRNA during oocyte maturation is regulated at the translational level by cytoplasmic polyadenylation. Two U-rich cytoplasmic polyadenylation elements (CPEs) in the 3' untranslated region of mouse c-mos mRNA are required for this process. The goal of this project is to isolate the factor(s) that bind to the CPEs of mouse c-mos mRNA and to determine the mechanism by which they activate polyadenylation and translation.
The CPE-binding factor (CPEB) will be isolated using several innovative approaches. Next, the binding of CPEB to c-mos mRNA and its role in polyadenylation will be analyzed. The RNA-binding domain and the effector domain of CPEB will be mapped. To investigate the mechanism of CPEB action, proteins interacting with its effector domain will be isolated using the yeast two-hybrid system. These proteins are expected to be involved in any of three processes: polyadenylation, translation or the regulation of meiosis, and are likely to establish links between these events.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences genetics heredity
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
69117 Heidelberg
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.