Objective
The project aims to identify transcriptional control elements of the human leucocyte common (CD45) antigen. Because of the large size of the human CD45 gene, we shall take advantage of the much smaller genome size of the Japanese puffer fish, Fugu rubripes. We shall clone the Fugu CD45 homologue by low stringency hybridisation screening of a splenic cDNA library or amplification of leucocyte cDNA using degenerate oligos. The Fugu CD45 cDNA will be used to probe a cosmid genomic library and the gene will be cloned and sequenced. Likely transcriptional control sequences will be identified by sequence analysis, subcloned into CAT vectors and tested for promoter and enhancer activity by transfection into Iymphoid and nonIymphoid lines. The human homologues of transcriptional control regions will be identified, cloned and tested by similar methods. Human tissue specific elements will also be tested in vivo by construction of transgenic mice.
Training content (objective, benefit and expected impact)
The proposed project will provide training in a range of molecular biology methods and in the analysis of gene expression using transient transfection assays and transgenic mice. It will provide information on the control of expression of an important gene product of leucocytes, perhaps Ieading to new insights into how differentiation of leucocytes is regulated.
Links with industry / industrial relevance (22)
The Edward Jenner Institute for Vaccine Research will discuss with Glaxo Wellcome the commercial exploitation of any patentable discoveries arising from this project.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine pharmacology and pharmacy pharmaceutical drugs vaccines
- natural sciences biological sciences genetics genomes
- natural sciences biological sciences molecular biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
RG20 7NN NEWBURY
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.