Objective
Research objectives and content
The envelope glycoproteins of the human and simian immunodeficiency viruses (HIV and SIV) are responsible for cell infection by mediating virus binding and entry. It is known that they are also important for late events in the replicative cycle in particular the assembly, budding and release of newly synthesised virions. It has been suggested that the cytoplasmic tail of the transmembrane component of the envelope glycoprotein can modulate the cellular location, cell surface expression and incorporation of envelope into viral particles. I propose to characterise the features of the protein that mediate these effects and investigate the interactions between the envelope glycoprotein cytoplasmic domain and other viral and cellular proteins. This will be done by analysing the intracellular transport of newly synthesised envelope glycoproteins and locating the sites of viral budding. I will perform morphological and biochemical analyses on the envelope glycoproteins (and mutants of these proteins) expressed in T cells and macrophages alone, or together with other viral structural proteins. For these studies, I will benefit from the expertise of the host institution in the cell biological analysis of virus-cell interactions, the use of novel expression techniques (e.g. Semliki Forest virus) and the use of green fluorescent protein as a means of protein tagging.
Training content (objective, benefit and expected impact)
I anticipate this project will lead to a clearer understanding of the assembly of HIV and SIV, and a better understanding membrane protein sorting mechanisms in general.
Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences health sciences infectious diseases viral diseases RNA viruses HIV
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
WC1E 6BT LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.