Objective
Research objectives and content
The main objective of this research is to investigate the NeuAc system. This would require the parallel subcloning of two plasmid-based E. coli genes for synthesis (N-acetyl neuraminic acid synthase = NeuS; EC 4.1.3.19) and activation (CMP-N-acetyl neuraminic acid synthetase = CMP-NeuS; EC 2.7.7.43) of neuraminic acid analogs into a commercial expression vector under the control of a strong inducible promoter (e.g. pKK223-3; Pharmacia), as well as the overexpression, purification, and characterization of the corresponding enzymes for preparative applications. Since only few ManNAc analogs are commercially available for the synthesis of sialic acid analogs the first step is to synthesize selected ManNAc derivatives or analogs. In this sense, it would be interesting the synthesis of the thio derivative: that can be very useful in the study of the NeuAc system and in the synthesis of 6-thio-N-acetyl-Dneuraminic acid derivatives.
Training content (objective, benefit and expected impact)
The synthesis of sialic acids analogues presents an important problem in view of the interesting biological properties that can be anticipated. As well as the synthetic and analytical techniques, with which I have become familiarized during my PhD, this work would include basic techniques of gene technology, protein chemistry, and enzyme-catalyzed synthesis of nucleotide-activated sugars and oligosaccharides, allowing me to use a wide range of methods.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
52074 AACHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.