Objective
The objective of the project is the use of multinuclear (1H, 13C, 15N) NMR spectroscopy to study the folding/unfolding process of paramagnetic metalloproteins such as c (horse heart, yeast) and b type (b5 from rat) cytochromes, their mutants and the isotopically enriched cyt b5 (rat). NMR spectroscopic techniques will be applied to partially unfolded states of the above proteins in order to probe: (i) the protein sites where the unfolding process starts; (ii) the dependence of the unfolding process on the redox state of the iron; (iii) the changes induced to the heme prosthetic group and their dependence on the presence (cyt c), or not (cyt b), of covalent links with the protein frame. The dynamical properties of the partially unfolded proteins will be studied by analyzing the exchange rates of amide protons and by performing MD calculations. The acquisition of J-correlated spectra using 500, 600, and 800 MHz magnetic fields will provide valuable information of the average structure of intermediate protein species exploiting the magnetic field dependence of nuclear resolution. The solution structures will be refined by using unconventional paramagnetic constraints for the paramagnetic systems. These constraints which are based on nuclear relaxation and hyperfine shifts (pseudocontact shifts) are long range iron nucleus constraints and are particularly useful in partially unfolded proteins.
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Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences physical sciences optics spectroscopy absorption spectroscopy
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
50121 Firenze
Italy
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.