Objective
Research objectives and content
The interaction between the fungus Cladosporium fulvum and its host tomato, provides an excellent model system to study the 'gene-for-gene' concept. The C. fulvum avirulence gene, Avr4, has been cloned and its product shown to induce a hypersensitive response (HR) in tomato genotypes that contain resistance gene Cf4. The aim of this proposal is to perform a structurefunction analyses on the AVR4 protein in order to determine the mechanism by which AVR4 interacts with its putative receptor in tomato. Constructs in which part of the Avr4 ORF is deleted will be expressed in Cf4 tomato genotypes, using the potato virus X expression system. Induction of HR will reveal which domains of the AVR4 protein are required for recognition by the host. Subsequent overproduction and radioactive labelling of active AVR4 peptides will allow the characterisation of putative AVR4 receptor sites in host cells.
Training content (objective, benefit and expected impact)
This project will provide training in gene manipulation and protein analysis techniques. In addition, important biological questions regarding pathogen recognition and induction of host defense responses in a gene-for-gene system will be addressed.
Links with industry / industrial relevance (22)
Elucidating molecular mechanisms by which plants recognise invading pathogens and activate defence processes is essential to allow molecular resistance breeding to a wide range of pathogens.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology virology
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences microbiology mycology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
6709 PD WAGENINGEN
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.