Objective
Research objectives and content
In bacterial conjugation, DNA is transferred from donor to recipient bacteria (or to yeast). In T-DNA transfer, structurally and functionally related to conjugation, DNA is transferred from Agrobacterium tumefaciens to plant cells. The aim of the proposed project is to investigate DNA processing during conjugation, using plasmid R388 as a model system. The relaxase protein TrwC is the key enzyme in this process. It is unique among relaxases since it catalyses intermolecular site-specific recombination between two oriT-containing DNA molecules. The biochemistry of TrwC will be analysed in detail, | to convert it to a technological tool. A second unique relaxase is VirD2, the relaxase of Agrobacrerium plasmids, that pilots the T-DNA to the plant cell nucleus. We propose to engineer TrwC by adding some features of VirD2 to achieve high frecuency site-specific DNA integration in yeast, plant and animal cells.Training content (objective, benefit and expected impact)
The trainee should become an expert in the biochemistry of relaxases. This technology should have an impact in genetic engineering projects using eukaryotic hosts. Integration of foreign DNA to specific genomic sites will be more efficient and | abolish differences in gene expression and stability common to alternative methods. Other biotechnological applications of these enzymes will be explored.Links with industry / industrial relevance (22)
None.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- medical and health sciences medical biotechnology genetic engineering
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
39011 SANTANDER
Spain
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.