Objective
Research objectives and content
The primary objective of this research is to bioengineer pancreatic beta cells suitable for mechanistic studies of enzyme regulation and later gene therapy of diabetes. At a first step, molecular biology techniques would be used to correct the impaired glucose-sensing apparatus of the popular RINm5F insulin-producing cell line. This would be achieved by overexpression of the two key elements responsible for beta cell glucose-recognition, glucokinase and the GLUT2 glucose transporter. This surrogate beta cell model, would also prove useful for in-depth studies of the regulation of lactate dehydrogenase activity. The overexpression of lactate dehydrogenase should provide a novel means of elucidating its importance in determining the fate of glucose metabolic flux towards mitochondrial oxidation, which is believed to becritical for insulin stimulus-secretion coupling. This innovative research project should yield a number of important new insights into the pancreatic beta cell stimulus-secretion coupling pathway, in addition to generating novel cell lines useful for gene therapy approaches and available for European experimental diabetes research.
Training content (objective, benefit and expected impact)
The purpose of this training is to gain practical expertise in a spectrum molecular biology techniques to complement biochemistry methods I acquired during my doctoral research. This training would provide me with all the necessary skills for a future career in experimental diabetes research. Links with industry / industrial relevance (22)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences medical biotechnology genetic engineering gene therapy
- natural sciences chemical sciences electrochemistry electrolysis
- medical and health sciences clinical medicine endocrinology diabetes
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
- natural sciences biological sciences molecular biology
You need to log in or register to use this function
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Data not available
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
30625 Hannover
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.