Objective
Research objectives and content
The AvrBs3 protein from phytopathogenic Xanthomonas bacteria triggers pepper plants carrying the resistance gene Bs3 to specifically recognize and counteract the pathogen. In susceptible tomato and pepper plants, however, AvrBs3 induceshypertrophy of leaf mesophyll cells. Both biological effects depend on the Xanthomonas Hrp type 111 secretion system that is thought to translocate bacterial proteins into the host cell. Furthermore, AvrBs3 has nuclear localization signals and a putative transcription activation domain that are required for its activity. Therefore, we hypothesize that AvrBs3 is a parasitic too allowing Xanthomonas to manipulate host gene expression. Target plant genes, of which expression is induced, will be isolated by cDNA-AFLP and their function studied by overexpression. Their promoter regions will be used in DNA-protein interaction assays to test whether AvrBs3 acts directly by binding DNA, or indirectly by interacting with host DNA binding proteins Training content (objective, benefit and expected impact)
This work involves using a broad spectrum of techniques in DNA / protein biochemistry and gene engineering, which will low me to aquire valuable skills for my research carreer.
Links with industry / industrial relevance (22)
The final aim of this basic research is to improve crop resistance to pathogens using new gene engineering strategies. The host laboratory has industrial links through an EC-Biotech project (contract no B104-CT972244)
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences microbiology bacteriology
- natural sciences biological sciences genetics DNA
- natural sciences biological sciences biochemistry biomolecules proteins
- agricultural sciences agriculture, forestry, and fisheries agriculture
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
06108 HALLE (SAALE)
Germany
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