Objective
Topoisomerase II is a nuclear enzyme required for cell division in all eukaryotes. In human cells, two distinct isozymes of topoisomerase II are expressed, termed a (170 kDa form) and D (180 kDa form). Both isozymes are known to be phosphoproteins in vivo. Topoisomerase IIa and B are also targets for inhibition by a range of the most commonly used anticancer drugs. We propose to investigate the functional significance of changes in the phosphorylation status of human topoisomerase IIa protein. These analyses will be conducted in two different systems: the budding yeast S. cerevisiae and human cell lines in tissue culture. Human topoisomerase IIa protein purified from yeast cells will be studied in comparison with mutant topoisomerase IIa proteins containing single amino acid substitutions eliminating key phosphoacceptor residues identified recently in the host laboratory. The mutated cDNAs encoding these phosphorylation site mutants will be overexpressed in human cells and dominant negative effects on cell cycle progression and faithful chromosome segregation analysed.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences chemical sciences organic chemistry amines
- natural sciences biological sciences genetics chromosomes
- natural sciences biological sciences biochemistry biomolecules proteins enzymes
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
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Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
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Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Funding Scheme
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Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
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Coordinator
54006 Thessaloniki
Greece
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.