Objectif Precise knowledge of the three dimensional (3D) structure of a given protein forms the basis of understanding its mechanism of action. Such knowledge is particularly important for designing and developing new drugs, but is also essential to understand the biology of humans and of other organisms. The HUGO-project has determined the complete human genome where the focus has now shifted from the DNA level to the structure and function of gene products at the protein level. An important method for protein structure determination is X-ray crystallography. The method requires access to single protein crystals, which has been the primary limiting step. This project aims to complete development of LEVMAC, a piece of equipment used in the field of Proteomics - to assist the crystallization of macromolecular structures, especially to resolve difficult cases where crystals of protein-ligand complexes are sought by the pharmaceutical industry. We will refine the prototype LEVMAC into an affordable and user-friendly instrument with good accuracy and validity, and with adequate software support. The LEVMAC device will be implemented in the frontline structural studies of naked and enveloped viruses, membrane receptor proteins, and protein-ligand complexes - areas in focus for advanced Proteomics and drug discovery. The equipment will allow structure determination of macromolecules to be reached faster and with higher accuracy than earlier, and at the same time waste less of precious macromolecular materials. To support these goals, the underlying algorithms for defining crystallization conditions will be further explored to provide nucleation phase diagrams. There is no competing equipment. Thus, the interest in the LEVMAC is expected to be very high among the pharmaceutical industries and within the scientific community. The proposed project will put CRAB at the forefront in the international Proteomics race and substantially benefit to the other participants as well. Champ scientifique medical and health scienceshealth sciencesinfectious diseasesRNA virusesnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicsnatural sciencesbiological sciencesmicrobiologyvirologynatural sciencesearth and related environmental sciencesgeologymineralogycrystallographynatural sciencesbiological sciencesgeneticsgenomes Programme(s) FP6-SME - Horizontal research activities involving SMEs: Specific activities covering wider field of research under the Focusing and Integrating Community Research programme 2002-2006. Thème(s) SME - Horizontal research activities involving SMEs Appel à propositions Data not available Régime de financement Cooperative - SMEs-Co-operative research contracts Coordinateur KAROLINSKA INSTITUTET Contribution de l’UE Aucune donnée Adresse NOBELS VAEG 5 STOCKHOLM Suède Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée Participants (4) Trier par ordre alphabétique Trier par contribution de l’UE Tout développer Tout réduire CRYSTAL RESEARCH AB Suède Contribution de l’UE Aucune donnée Adresse VAESTMANNAGATAN 52 STOCKHOLM Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée FATMAN BIOINFORMATIONAL DESIGNS OY Finlande Contribution de l’UE Aucune donnée Adresse Lemminkäisenkatu 14 A U, FI-20520, Turku TURKU Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée OÜ LEAB EESTI Estonie Contribution de l’UE Aucune donnée Adresse Laki 12 TALLINN Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée UNIVERSITÄT BASEL Suisse Contribution de l’UE Aucune donnée Adresse Petersgraben 35 BASEL Voir sur la carte Liens Site web Opens in new window Coût total Aucune donnée