Objective
The storage of triacylglycerols so as to provide energy for the organism in case of food deprivation is amajor function of adipocytes. In addition, the adipose tissue contains the largest body pool of free-cholesterol localized in the lipid droplet. Moreover, the adipose tissue constitutes the highest expressing tissue of caveolinswhich are known to act as cholesterol-binding proteins. We propose to try to find out if the dynamics of thesecaveolins, that have recently been reported to be around lipid droplets, could be implicated in the transport/storage of cholesterol to the lipid droplets. For this purpose, we will follow the distribution of cholesterol analogy like filliping and dehydroergsoterolduring the time-course of 3T3-L1 adiposities and co localize it with specific adiposity markers tagged with fluorescent protein. Besides, by using [3H] photo cholesterol, it will be possible to cross-link proteins interacting directly with cholesterol. In order to know if cavalla dynamics could influence cholesterol trafficking, we will study the consequences of an over expression of capelins on cholesterol distribution and cholesterol efflux. To establish the physiological relevance of capelins in cholesterol trafficking, we will try to find out if caveolin-1null mice generated in the host lab (Drab et al, 2001) are resistant to high fat diet as reported for another strain. What is more, in vitro differentiation of these preadipocytes will give us a physiological model of knock-outcaveolin-1 in fat cells to study the effect on cholesterol distribution. Then, we will analyse the effect of adownregulation of capelins on cholesterol distribution by using Kenai in 3T3-L1 adiposities. In parallel, overexpression of different parts of cave Olin tagged with fluorescent proteins will be achieved in order to determine the effect on cholesterol localization. Finally, we propose to study the consequences of a down regulation of Niemann Pick C-I (NPC1) by RNAi, #
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences biochemistry biomolecules lipids
- medical and health sciences health sciences nutrition
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
DRESDEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.