Objective The ANGIOSKIN consortium wants to bring the proof of concept that therapeutic genes can be safely delivered to skin by DNA electrotransfer (electrogenetherapy) in order to prevent or to treat acquired or inherited skin diseases. ANGIOSKIN proposal is based on the results of the 5th FP CLINIPORATOR project (analysis of the mechanisms of DNA electrotransfer and elaboration of a CE labelled pulse generator) and of the 5th FP ESOPE project (preparation of the Standard Operating Procedures of Electrogenetherapy, electrotransferring a reporter gene in humans), and on a proprietary gene coding for a potent human antiangiogenic factor. This factor is the disintegrin fragment of the Metargidin, also referred as AMD-15 (MDC 15 in mouse), and it specifically binds to t he avß3 and a5ß1 integrins involved in angiogenic processes. ANGIOSKIN consortium will: - Electrotransfer the proprietary therapeutic antiangiogenesis gene to melanoma cutaneous metastasis in humans, using the procedures validated in ESOPE project, to show its clinical efficacy; non invasive biophysical methods (e.g. Doppler ultrasonography) will be used to monitor the angioangiogenic effects; - Develop new specific electrodes for skin lesions treatment, based on arrays of hollow microneedles allowing both DNA and electric pulse delivery, and requiring the use of micromechanics ans microfluidics techniques; - Validate the electrodes (safety, efficacy) on normal skin in animals, and analyse the effects of the electrotransfer of the antioangiogenetic factor on models of skin disease related to excessive angiogenesis (models of psoriasis), using non nonvasive biophysical as well as histological methods to follow changes in the vascularization of the lesion; - Finally, electrotransfer the therapeutic gene to a be nign lesion (psoriasis) in humans as a proof of concept of the use of non-viral gene therapy to treat acquired or inherited skin diseases. Fields of science natural sciencesphysical sciencesclassical mechanicsfluid mechanicsmicrofluidicsmedical and health sciencesmedical biotechnologygenetic engineeringgene therapynatural sciencesbiological sciencesgeneticsDNAmedical and health sciencesclinical medicineoncologyskin cancermelanoma Keywords DNA electrotransfer electroporation medical instrumentation naked DNA non-viral gene therapy Programme(s) FP6-LIFESCIHEALTH - Life sciences, genomics and biotechnology for health: Thematic Priority 1 under the Focusing and Integrating Community Research programme 2002-2006. Topic(s) LSH-2003-1.2.4-8 - Use of gene transfer for curative therapy of human skin disease Call for proposal FP6-2003-LIFESCIHEALTH-I See other projects for this call Funding Scheme STIP - Specific Targeted Innovation Project Coordinator CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE - DÉLÉGATION ILE-DE-FRANCE EST EU contribution No data Address Tour Europa 126 THIAIS France See on map Links Website Opens in new window Total cost No data Participants (9) Sort alphabetically Sort by EU Contribution Expand all Collapse all IGEA S.R.L. Italy EU contribution No data Address VIA PARMENIDE 10/A CARPI (MO) See on map Links Website Opens in new window Total cost No data INSTITUT GUSTAVE-ROUSSY France EU contribution No data Address 39 rue Camille Desmoulins VILLEJUIF See on map Links Website Opens in new window Total cost No data DEPARTMENT OF ONCOLOGY, HERLEV HOSPITAL Denmark EU contribution No data Address Herlev Ringvej 75 HERLEV See on map Links Website Opens in new window Total cost No data BAYERISCHE JULIUS-MAXIMILIANS-UNIVERSITAET WUERZBURG Germany EU contribution No data Address Sanderring 2 WUERZBURG See on map Links Website Opens in new window Total cost No data BIOALLIANCE PHARMA SA France EU contribution No data Address 59 boulevard General Martial Valin PARIS See on map Links Website Opens in new window Total cost No data UNIVERSITY OF LJUBLJANA, FACULTY OF ELECTRICAL ENGINEERING Slovenia EU contribution No data Address Trzaska 25 LJUBLJANA See on map Links Website Opens in new window Total cost No data DEPT. OF DERMATOLOGY, GENTOFTE UNIVERSITY HOSPITAL, COPENHAGEN, DENMARK Denmark EU contribution No data Address Niels Andersens Vej 65 HELLERUP See on map Links Website Opens in new window Total cost No data UNIVERSITÄTSMEDIZIN GÖTTINGEN - GEORG-AUGUST-UNIVERSITÄT GÖTTINGEN - STIFTUNG ÖFFENTLICHEN RECHTS Germany EU contribution No data Address Robert-Koch-Str. 40 GÖTTINGEN See on map Links Website Opens in new window Total cost No data UNIVERSITÉ CATHOLIQUE DE LOUVAIN Belgium EU contribution No data Address Place de l'Université 1' LOUVAIN-LA-NEUVE See on map Links Website Opens in new window Total cost No data
