Objective
The basic aim of this proposal is to investigate the molecular mechanisms of fast and slow endocytosis at the synapse. We will generate stable lines of zebrafish expressing fluorescent proteins thought to be involved in endocytosis under control of neuron al promoters; then use TIRFM to monitor recruitment of these proteins to the presynaptic membrane in the giant synaptic terminal of retinal bipolar cells. In particular, we will produce zebrafish lines expressing the fluorescent fusion proteins clathrin-l ight-chain-a-monomeric red fluorescent protein (clathrin-mRFP), endophilin-mRFP, amphiphysin-mRFP and synaptopHluorin a pH sensitive GFP related reporter of exocytosis and endocytosis. We will test the hypothesis that endophilin is involved in fast endocyt osis at the synapse, while clathrin and amphiphysin mediate slow but not fast endocytosis. These transgenic zebrafish lines may also be used to monitor exocytosis and endocytosis in intact neural circuits, such as the retina, using multi-photon confocal mi croscopy.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- medical and health sciences clinical medicine ophthalmology
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Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2004-MOBILITY-5
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
LONDON
United Kingdom
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.