Objective
This proposal seeks to identify potential prion proteins within the model plant Arabidopsis thaliana.The term prion was first used to describe the infectious agent causing scrapie, a transmissiblespongiform encephalopathy (TSE) disease, in sheep. Since the n TSE's have been identified in cattle (BSE) and an association between BSE and a variant of a human TSE, Cruetzfeld-Jakob disease (CJD),have been established. The identification of prion-like proteins in Saccharomyces cerevisiae has opened up a new era in t he study of prion proteins.
As well as redefining the term prion to encompass an array of proteins that can behave in an infectious manner, the discovery of prions in yeast allows detailed genetic analysis to be carried out on factors affecting prion stability and propagation using well-established yeast genetic techniques. Furthermore, studies of yeast prions suggest that the prion phenomena may well be widespread throughout nature and that prions have the ability to carry out normal cellular functions. Prionisation domains have been identified and have been shown to be transferable. Using bio-informatics techniques a number of plant proteins that contain putative prion domains have identified. The experimental approach to be taken in this proposal is to transfer these potential plant prion domains and fuse them with green fluorescent protein (GFP) and also use them to replace the prion domain of the known yeast prion protein Sup35. Prions in yeast form aggregates.
We will therefore monitor the aggregation state of GF P in the constructs and observe if they behave like known yeast prions. We will also carry out detailed yeast genetics to investigate whether the plant prion domains can substitute for the natural prion domain of the Sup35 protein. In addition, we will create and screen an Arabidopsis prion domain library constructed from random plant amino acid sequences fused to a yeast selectable marker. Yeast
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences genetics
- natural sciences biological sciences biochemistry biomolecules proteins
- agricultural sciences animal and dairy science domestic animals animal husbandry
- natural sciences chemical sciences organic chemistry amines
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP6-2002-MOBILITY-12
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
IRG - Marie Curie actions-International re-integration grants
Coordinator
MAYNOOTH, CO. KILDARE
Ireland
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