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Content archived on 2024-05-29

Exploring the Potential of Microfluidics and Compartmentalisation for Selections in Directed Evolution

Objective

Miniaturisation has become a driver of technological and scientific advance for problems that can only be solved by combinatorial approaches, requiring comprehensive testing of each member of a library.

We now plan to develop a new technology based on the idea of minimising the volume of biological and chemical reactions further.

Normally, each experiment in biology or chemistry is performed in a tube or multi-well plate. We now want to perform performed each assay in a separate microscopic droplet, with a volume one billion times smaller than a conventional assay.

These droplets will be formed, manipulated and analysed in a microfluidic system, consisting of a network of microscopic channels through which the droplets will flow in a stream of oil.

We aim to demonstrate their utility of this technology to search large numbers of compounds to develop new catalysts in directed evolution experiments.

Fields of science (EuroSciVoc)

Keywords

Topic(s)

Call for proposal

FP6-2002-MOBILITY-5
See other projects for this call

Funding Scheme

EIF - Marie Curie actions-Intra-European Fellowships

Coordinator

THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
EU contribution
No data
Address


United Kingdom
Total cost
No data