Objective
In eukaryotes, nuclear pore complexes (NPCs) are large assemblies that selectively transport cargoes across the nuclear envelope. Determination of the architecture of the NPC in molecular detail is central to understanding nuclear transport. Structural analysis of the NPC is a major challenge given its sheer size and its dynamic nature. Cryo-electron tomography (cryo-ET) has successfully been used to elucidate the overall architecture of the NPC, but substantial improvements in resolution are needed to use cryo-ET maps as a scaffold into which high-resolution structures of individual nucleoporins (Nups) or subdomains can be fitted reliably. We propose to achieve such goal by using a novel technique for cryo-sectioning of frozen hydrated biological samples, focused ion beam (FIB) milling, to obtain thin sections of nuclei in lifelike conditions, that will permit us to achieve cryo-ET maps of 2-3nm. Furthermore, we will use metallothionein tags, that nucleate gold clusters, in order to localize individual Nups with high-accuracy within the NPC map. These data, together with cross linking data from mass spectroscopy to determine distances between pairs of Nups, available crystallographic structures of single Nups, and cryo-EM data of subdomains, will be integrated into a high-resolution structure of the entire NPC using a proteomics approach.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins proteomics
- natural sciences physical sciences optics microscopy electron microscopy
- natural sciences physical sciences optics spectroscopy
You need to log in or register to use this function
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-IIF-2008
See other projects for this call
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
80539 MUNCHEN
Germany
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.