Objetivo Single molecules based techniques provide the ultimate toolkit to study complex biological systems. In single-molecule approaches, molecules do not need to be synchronised as in ensemble studies, and rare and/or transient species along a reaction pathway as well as heterogeneity and disorder in a sample can be revealed.Observing and manipulating single molecules, however, is generally complicated, time consuming and withstand several technical limitations that hamper the study of single enzymes to a few selected examples.Here I am proposing to develop a new technology to study single native enzymes that is sensitive, simple and inexpensive, and has a temporal resolution ranging from few ¼seconds to hours.Nanopores have been used to detect single molecules and to investigate mechanisms of chemical reactions at the single molecule level. The basic concept of nanopore analysis is to observe, under an applied potential, the disruption of the flow of ions through the pore caused by the interaction of the molecules of interest with a binding site within the pore.Similarly, small enzymes or functional nucleic acids will be attached to the vestibule of a biological nanopore via disulfide bridge or click chemistry. The conformational changes associated with catalysis will then be observed by the altered ionic flow through the pore. In addition, when a charged chaotropic agent is placed on the trans side of the bilayer, the applied potential will allow the directional control of the flow of the chaotropic agent through the pore that, ultimately, also will control the unfolding and refolding of the protein attached to the pore. This will allow investigations of reversible unfolding processes far from equilibrium at the single molecule level for the first time. Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acidsnatural scienceschemical sciencescatalysisnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-SG-LS9 - ERC Starting Grant - Applied life sciences and biotechnology Convocatoria de propuestas ERC-2010-StG_20091118 Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-SG - ERC Starting Grant Institución de acogida RIJKSUNIVERSITEIT GRONINGEN Aportación de la UE € 451 215,26 Dirección Broerstraat 5 9712CP Groningen Países Bajos Ver en el mapa Región Noord-Nederland Groningen Overig Groningen Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Dick Veldhuis (Dr.) Investigador principal Giovanni Maglia (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (2) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo RIJKSUNIVERSITEIT GRONINGEN Países Bajos Aportación de la UE € 451 215,26 Dirección Broerstraat 5 9712CP Groningen Ver en el mapa Región Noord-Nederland Groningen Overig Groningen Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Dick Veldhuis (Dr.) Investigador principal Giovanni Maglia (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos KATHOLIEKE UNIVERSITEIT LEUVEN La participación finalizó Bélgica Aportación de la UE € 769 258,34 Dirección OUDE MARKT 13 3000 Leuven Ver en el mapa Región Vlaams Gewest Prov. Vlaams-Brabant Arr. Leuven Tipo de actividad Higher or Secondary Education Establishments Contacto administrativo Myriam Witvrouw (Ms.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos
