Objetivo The protein and lipid composition of the cell plasma membrane is quite heterogeneous. In Saccharomyces cerevisiae, the arginine permease Can1 and other plasma membrane proteins, show a “patchy” distribution, being enriched in the MCC (Membrane Compartment of Can1) subdomain. The formation and maintenance of this compartment depends on the transmembrane protein Nce102 and the cytosolic protein Pil1. The latest is responsible for the formation of cytosolic protein complexes, the eisosomes, in vicinity to MCC patches. Sur7 and Lsp1 are also structural components of the MCC and eisosome, respectively. MCC and eisosomal proteins are conserved in fungi and have been linked to the regulation of endocytosis, sphingolipid signaling, cell wall synthesis, plasma membrane organization and cell cycle. Their exact involvement in each of these processes remains largely elusive, and differences have been observed among fungal species. Interestingly, fungal cells affected in the eisosomal/MCC machinery show altered sensitivity to antifungal drugs. In the present proposal, the filamentous fungus Aspergillus nidulans will be used as a model system for the study of eisosomal/MCC proteins in endocytosis of plasma membrane lipids and proteins, cell wall homeostasis, membrane organization and cell cycle progression. The objectives of the project consist on the cloning of eisosomal genes, the study of their transcriptional regulation, and the effects of their deletion on different cellular functions. The subcellular localization of the respective proteins will also be addressed. Specific emphasis will be given on the involvement of eisosomal proteins in cell wall synthesis and amino acid transporter post-translational regulation, based on the results from previous work in the host laboratory. Genetic and proteomic tools will be further used for the identification of new partners and the structure-function analysis of eisosomal proteins. Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesmicrobiologymycologynatural sciencesbiological sciencesbiochemistrybiomoleculeslipidsnatural scienceschemical sciencesorganic chemistryaminesmedical and health sciencesbasic medicinephysiologyhomeostasis Programa(s) FP7-PEOPLE - Specific programme "People" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) FP7-PEOPLE-2009-RG - Marie Curie Action: "Reintegration Grants" Convocatoria de propuestas FP7-PEOPLE-2009-RG Consulte otros proyectos de esta convocatoria Régimen de financiación MC-ERG - European Re-integration Grants (ERG) Coordinador "NATIONAL CENTER FOR SCIENTIFIC RESEARCH ""DEMOKRITOS""" Aportación de la UE € 45 000,00 Dirección END OF PATRIARCHOU GRIGORIOU E AND 27 NEAPOLEOS STREET 15341 Agia Paraskevi Grecia Ver en el mapa Región Αττική Aττική Βόρειος Τομέας Αθηνών Tipo de actividad Research Organisations Contacto administrativo Vassiliki Sophianopoulou (Dr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos
