Objective
The protein and lipid composition of the cell plasma membrane is quite heterogeneous. In Saccharomyces cerevisiae, the arginine permease Can1 and other plasma membrane proteins, show a “patchy” distribution, being enriched in the MCC (Membrane Compartment of Can1) subdomain. The formation and maintenance of this compartment depends on the transmembrane protein Nce102 and the cytosolic protein Pil1. The latest is responsible for the formation of cytosolic protein complexes, the eisosomes, in vicinity to MCC patches. Sur7 and Lsp1 are also structural components of the MCC and eisosome, respectively. MCC and eisosomal proteins are conserved in fungi and have been linked to the regulation of endocytosis, sphingolipid signaling, cell wall synthesis, plasma membrane organization and cell cycle. Their exact involvement in each of these processes remains largely elusive, and differences have been observed among fungal species. Interestingly, fungal cells affected in the eisosomal/MCC machinery show altered sensitivity to antifungal drugs. In the present proposal, the filamentous fungus Aspergillus nidulans will be used as a model system for the study of eisosomal/MCC proteins in endocytosis of plasma membrane lipids and proteins, cell wall homeostasis, membrane organization and cell cycle progression. The objectives of the project consist on the cloning of eisosomal genes, the study of their transcriptional regulation, and the effects of their deletion on different cellular functions. The subcellular localization of the respective proteins will also be addressed. Specific emphasis will be given on the involvement of eisosomal proteins in cell wall synthesis and amino acid transporter post-translational regulation, based on the results from previous work in the host laboratory. Genetic and proteomic tools will be further used for the identification of new partners and the structure-function analysis of eisosomal proteins.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences microbiology mycology
- natural sciences biological sciences biochemistry biomolecules lipids
- natural sciences chemical sciences organic chemistry amines
- medical and health sciences basic medicine physiology homeostasis
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2009-RG
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
153 41 AGIA PARASKEVI
Greece
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.