Objective
Crystal structures of beta1 and beta2 adrenergic G protein-coupled receptors (GPCRs) have been determined recently. We propose to extend the structural information about this physiologically and medically important GPCR subfamily by obtaining the structures of the beta3 and the alpha adrenergic receptors. The ample experience in GPCR structural biology of the host laboratory, complemented by its multidisciplinary approach, provides an ideal environment for this challenging task. We will make use of high-throughput facilities and methods to optimize the receptor constructs for expression and protein stability. We will use various crystallization methods and available robotic nano-liter dispensers to determine suitable conditions for crystal growth. We will have access to state-of-the-art microcrystallography beamlines at the Swiss Light Source synchrotron at Paul Scherrer Institut (PSI) to test a large number of obtained crystals and collect the best possible diffraction datasets. Once diffraction data of sufficient quality has been obtained, the structure will be solved by molecular replacement. In collaboration with other PSI scientists and external academic and industrial partners we will use the structural results obtained for the adrenergic receptor subtypes to study the structural basis of ligand efficacy, i.e. how the process of ligand binding is translated into receptor function, and to find applications in therapeutic drug development.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- medical and health sciences basic medicine pharmacology and pharmacy drug discovery
- engineering and technology materials engineering crystals
- natural sciences biological sciences biochemistry biomolecules proteins
- natural sciences biological sciences molecular biology structural biology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
FP7-PEOPLE-2010-IEF
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Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Coordinator
5232 VILLIGEN PSI
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.