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CORDIS

Fast and Sensitive Detection of Plant Hormones by Ion Mobility Spectrometry

Project description

Better detection of plant hormones may bring about major advances in agriculture

Plant hormones (PHs) play a crucial role in plant growth, seed germination, fruit maturation and fruit ripening. Hence, their detection is important for plant physiology studies and applications in agriculture. Conventional PH detection techniques use high-performance liquid and gas chromatography, and their combination with mass spectrometry. However, these techniques are time consuming, whereas ion mobility spectrometry has the potential to be a fast and sensitive method of detection for PHs. The EU-funded PlantIMS project will apply the IMS technique to PH detection and optimise its operation parameters to improve sensitivity and selectivity. The research fellow will thus develop sampling methodologies for the detection of PHs and for online monitoring.

Objective

The Plant Hormones (PHs) influence the plant growth, seed germination, fruit maturation, and fruit ripening, hence they play significant roles in the production of food crops. The detection of PHs is thus of great importance for the plant physiology studies and for research and applications in agriculture. The conventional PHs detection techniques employ mainly high-performance liquid and gas chromatography (HPLC, GC) and their combination with mass spectrometry. These techniques are reliable and sensitive; however, time demanding and require complex sample preparation methods. The Ion Mobility Spectrometry (IMS) has the potential to be developed as a fast and sensitive detection method for PHs. The objective of this project is to apply the IMS technique for PHs detection. Within the project, we plan to optimize operation parameters of IMS to improve the sensitivity and selectivity, and also to develop suitable sampling methods and methodology for detection of the PHs from plants and for online monitoring. The host institution (HI) offers an excellent multidisciplinary research environment and enables the fellow access to their unique experimental techniques in the field of IMS instrumentation including IMS-Time of Flight Mass Spectrometry (IMS-TOFMS). In addition, HI will provide expertise in the field of ion chemistry, plasma physics, access to available analytical methods, and interdisciplinary collaboration in the field of plant physiology. The fellow will contribute with her unique experience in the application of IMS in analytical sciences, to develop the sampling methods and methodology of IMS measurements of PHs. The project will boost the fellow’s research skills, enables her to gain experience in interdisciplinary research, and start an independent researcher career.

Coordinator

UNIVERZITA KOMENSKEHO V BRATISLAVE
Net EU contribution
€ 155 364,48
Address
SAFARIKOVO NAM 6
814 99 Bratislava
Slovakia

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Region
Slovensko Bratislavský kraj Bratislavský kraj
Activity type
Higher or Secondary Education Establishments
Links
Total cost
€ 155 364,48