Objective
FASTR 3D SIM: Focus on Advancing the Spatial and Temporal Resolution of 3D Structured Illumination Microscopy
Super-resolved structured illumination microscopy (SR-SIM) is an important method to surpass the resolution limit in fluorescence microscopy. SR-SIM is compatible with many fluorescent labels and ensures low photo-damage, making it an ideal candidate for live-cell imaging. However, to be truly applicable, very fast 3D imaging has to be realized, as the cellular structuring is 3D in nature and fast dynamics have to be captured. In addition, many biological processes occur at length scales below 100nm, beyond SR-SIMs two-fold resolution improvement.
I will develop an advanced 3D SR-SIM microscope able to capture 3D images at video rate, i.e. 25 3D images per second, 10 times quicker than the fastest currently available systems. The extreme speed-up is achieved by a unique multi-focal optics approach available at the host institute, and will also reduce the photo-toxicity to the sample. My SIM reconstruction code (www.fairsim.org) will be extended to 3D imaging, and include new algorithms tackling low light levels and strong out-of-focus light.
I will also develop a revolutionary combination of SIM with super-resolution optical fluctuation imaging (SOFI), a specialty of the Dedecker lab. SOFI is employed to create non-linear effects, allowing SIM to surpass its 2x resolution enhancement. This enables rapid and repeatable 3D imaging of cells with a spatial resolution approx. 60 nm in x,y and 150 nm in z.
I will apply the tools to an acute biological question, the dynamics of mitochondrial and ER interactions, used by the cell as signaling scaffolding points. Here both the temporal and spatial resolution of the proposed system is required to observe the very fast dynamics.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: https://op.europa.eu/en/web/eu-vocabularies/euroscivoc.
- natural sciences computer and information sciences software
- engineering and technology electrical engineering, electronic engineering, information engineering electronic engineering sensors optical sensors
- natural sciences biological sciences genetics DNA
- natural sciences physical sciences optics microscopy
- natural sciences computer and information sciences data science data processing
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2016
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
3000 Leuven
Belgium
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.