Directed Evolution of Artificial Metalloenzymes for In Vivo Applications

Project Information

DrEAM

Grant agreement ID: 694424

DOI 10.3030/694424

Project closed

EC signature date 18 August 2016

Start date 1 October 2016

End date 30 September 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 490 700,00

EU contribution € 2 490 700,00

Coordinated by

UNIVERSITAT BASEL
Switzerland

Publications

Peer reviewed articles (29)

A Dual Anchoring Strategy for the Directed Evolution of Improved Artificial Transfer Hydrogenases Based on Carbonic Anhydrase.

Author(s): Alina Stein; Dongping Chen; Nico V. Igareta; Yoann Cotelle; Johannes G. Rebelein; Thomas R. Ward
Published in: ACS Central Science, Issue 23747943, 2021, ISSN 2374-7943
Publisher: American Chemical Society
DOI: 10.1021/acscentsci.1c00825

Bioorthogonal strategies for the in vivo synthesis or release of drugs

Author(s): Boris Lozhkin, Thomas R.Ward
Published in: Bioorganic & Medicinal Chemistry, Issue 09680896, 2021, ISSN 0968-0896
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.bmc.2021.116310

Functional and morphological adaptation in DNA protocells via signal processing prompted by artificial metalloenzymes

Author(s): Avik Samanta, Valerio Sabatino, Thomas R. Ward, Andreas Walther
Published in: Nature Nanotechnology, Issue 15/11, 2020, Page(s) 914-921, ISSN 1748-3387
Publisher: Nature Publishing Group
DOI: 10.1038/s41565-020-0761-y

Enantioselective Hydroxylation of Benzylic C(sp 3 )–H Bonds by an Artificial Iron Hydroxylase Based on the Biotin–Streptavidin Technology

Author(s): Joan Serrano-Plana, Corentin Rumo, Johannes G. Rebelein, Ryan L. Peterson, Maxime Barnet, Thomas R. Ward
Published in: Journal of the American Chemical Society, Issue 142/24, 2020, Page(s) 10617-10623, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c02788

Directed Evolution of a Surface-Displayed Artificial Allylic Deallylase Relying on a GFP Reporter Protein

Author(s): Alain Baiyoumy; Jaicy Vallapurackal; Fabian Schwizer; Tillmann Heinisch; Tsvetan Kardashliev; Martin Held; Sven Panke; Thomas R. Ward
Published in: ACS Catalysis, Issue 21555435, 2021, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.3929/ethz-b-000506507

An Artificial Metalloenzyme Based on a Copper Heteroscorpionate Enables sp3 C–H Functionalization via Intramolecular Carbene Insertion

Author(s): Corentin Rumo, Alina Stein, Juliane Klehr, Ryo Tachibana, Alessandro Prescimone, Daniel Häussinger, Thomas R. Ward
Published in: Journal of the American Chemical Society, Issue 00027863, 2022, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.2c03311

Engineering a Metathesis-Catalyzing Artificial Metalloenzyme Based on HaloTag

Author(s): Sandro Fischer, Thomas R. Ward, and Alexandria D. Liang
Published in: ACS Catalysis, Issue 21555435, 2021, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.1c01470

Droplet Microfluidics and Directed Evolution of Enzymes: an Intertwined Journey

Author(s): Ariane Stucki, Jaicy Vallapurackal, Thomas R. Ward, Petra S Dittrich
Published in: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202016154

Design and evolution of chimeric streptavidin for protein-enabled dual gold catalysis

Author(s): Fadri Christoffel, Nico V. Igareta, Michela M. Pellizzoni, Laura Tiessler-Sala, Boris Lozhkin, Daniel C. Spiess, Agustí Lledós, Jean-Didier Maréchal, Ryan L. Peterson, Thomas R. Ward
Published in: Nature Catalysis, Issue 25201158, 2021, ISSN 2520-1158
Publisher: Nature Publishing Group
DOI: 10.1038/s41929-021-00651-9

Chemo-enzymatic cascades to produce cycloalkenes from bio-based resources

Author(s): Shuke Wu, Yi Zhou, Daniel Gerngross, Markus Jeschek, Thomas R. Ward
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-13071-y

Streptavidin-Enzyme Linked Aggregates for the One-Step Assembly and Purification of Enzyme Cascades

Author(s): Hendrik Mallin, Thomas R. Ward
Published in: ChemCatChem, Issue 10, 2018, Page(s) 2810 –2816, ISSN 1867-3880
Publisher: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.201800162

Photo-Driven Hydrogen Evolution by an Artificial Hydrogenase Utilizing the Biotin-Streptavidin Technology

Author(s): Sascha G. Keller, Benjamin Probst, Tillmann Heinisch, Roger Alberto, Thomas R. Ward
Published in: Helvetica Chimica Acta, Issue 101/4, 2018, Page(s) e1800036, ISSN 0018-019X
Publisher: Verlag Helvetica Chimica Acta
DOI: 10.1002/hlca.201800036

Streptavidin as a Scaffold for Light-Induced Long-Lived Charge Separation

Author(s): Sascha G. Keller, Andrea Pannwitz, Hendrik Mallin, Oliver S. Wenger, Thomas R. Ward
Published in: Chemistry - A European Journal, Issue 23/71, 2017, Page(s) 18019-18024, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201703885

In vivo catalyzed new-to-nature reactions

Author(s): Johannes G Rebelein, Thomas R Ward
Published in: Current Opinion in Biotechnology, Issue 53, 2018, Page(s) 106-114, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2017.12.008

Directed Evolution of an Artificial Imine Reductase

Author(s): Martina Hestericová, Tillman Heinisch, Lur Alonso-Cotchico, Jean-Didier Maréchal, Pietro Vidossich, Thomas R. Ward
Published in: Angewandte Chemie International Edition, Issue 57/7, 2018, Page(s) 1863-1868, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201711016

A cell-penetrating artificial metalloenzyme regulates a gene switch in a designer mammalian cell

Author(s): Yasunori Okamoto, Ryosuke Kojima, Fabian Schwizer, Eline Bartolami, Tillmann Heinisch, Stefan Matile, Martin Fussenegger, Thomas R. Ward
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-04440-0

An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin

Author(s): Jingming Zhao, Daniel G. Bachmann, Markus Lenz, Dennis G. Gillingham, Thomas R. Ward
Published in: Catalysis Science & Technology, Issue 8/9, 2018, Page(s) 2294-2298, ISSN 2044-4753
Publisher: Royal Society of Chemistry
DOI: 10.1039/C8CY00646F

Cross-Regulation of an Artificial Metalloenzyme

Author(s): Yasunori Okamoto, Thomas R. Ward
Published in: Angewandte Chemie International Edition, Issue 56/34, 2017, Page(s) 10156-10160, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201702181

Ferritin encapsulation of artificial metalloenzymes: engineering a tertiary coordination sphere for an artificial transfer hydrogenase

Author(s): Martina Hestericová, Tillmann Heinisch, Markus Lenz, Thomas R. Ward
Published in: Dalton Transactions, Issue 47/32, 2018, Page(s) 10837-10841, ISSN 1477-9226
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8dt02224k

Genetic Engineering of an Artificial Metalloenzyme for Transfer Hydrogenation of a Self-Immolative Substrate in Escherichia coli’ s Periplasm

Author(s): Jingming Zhao, Johannes G. Rebelein, Hendrik Mallin, Christian Trindler, Michela M. Pellizzoni, Thomas R. Ward
Published in: Journal of the American Chemical Society, Issue 140/41, 2018, Page(s) 13171-13175, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.8b07189

Chemical Optimization of Whole-Cell Transfer Hydrogenation Using Carbonic Anhydrase as Host Protein

Author(s): Johannes G. Rebelein, Yoann Cotelle, Brett Garabedian, Thomas R. Ward
Published in: ACS Catalysis, Issue 9/5, 2019, Page(s) 4173-4178, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.9b01006

Artificial Metalloenzymes Based on the Biotin–Streptavidin Technology: Enzymatic Cascades and Directed Evolution

Author(s): Alexandria Deliz Liang, Joan Serrano-Plana, Ryan L. Peterson, Thomas R. Ward
Published in: Accounts of Chemical Research, Issue 52/3, 2019, Page(s) 585-595, ISSN 0001-4842
Publisher: American Chemical Society
DOI: 10.1021/acs.accounts.8b00618

E. coli surface display of streptavidin for directed evolution of an allylic deallylase

Author(s): Tillmann Heinisch, Fabian Schwizer, Brett Garabedian, Eszter Csibra, Markus Jeschek, Jaicy Vallapurackal, Vitor B. Pinheiro, Philippe Marlière, Sven Panke, Thomas R. Ward
Published in: Chemical Science, Issue 9/24, 2018, Page(s) 5383-5388, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8sc00484f

Artificial Metalloenzymes: Challenges and Opportunities

Author(s): Holly J. Davis, Thomas R. Ward
Published in: ACS Central Science, Issue 5/7, 2019, Page(s) 1120-1136, ISSN 2374-7943
Publisher: American Chemical Society
DOI: 10.1021/acscentsci.9b00397

“Close-to-Release”: Spontaneous Bioorthogonal Uncaging Resulting from Ring-Closing Metathesis

Author(s): Valerio Sabatino, Johannes G. Rebelein, Thomas R. Ward
Published in: Journal of the American Chemical Society, Issue 141/43, 2019, Page(s) 17048-17052, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.9b07193

Breaking Symmetry: Engineering Single-Chain Dimeric Streptavidin as Host for Artificial Metalloenzymes

Author(s): Shuke Wu, Yi Zhou, Johannes G. Rebelein, Miriam Kuhn, Hendrik Mallin, Jingming Zhao, Nico V. Igareta, Thomas R. Ward
Published in: Journal of the American Chemical Society, Issue 141/40, 2019, Page(s) 15869-15878, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.9b06923

Incorporation of metal-chelating unnatural amino acids into halotag for allylic deamination

Author(s): Alina Stein, Alexandria Deliz Liang, Reyhan Sahin, Thomas R.Ward
Published in: Journal of Organometallic Chemistry, Issue 0022328X, 2022, ISSN 0022-328X
Publisher: Elsevier BV
DOI: 10.1016/j.jorganchem.2022.122272

Synthesis of N-Substituted Indoles via Aqueous Ring-Closing Metathesis

Author(s): Valerio Sabatino, Dario Staub, Thomas R. Ward
Published in: Catalysis Letters, Issue 151/1, 2021, Page(s) 1-7, ISSN 1011-372X
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10562-020-03271-3

Ultrahigh-Throughput Screening of an Artificial Metalloenzyme using Double Emulsions

Author(s): Jaicy Vallapurackal, Ariane Stucki, Alexandria Deliz Liang, Juliane Klehr, Petra S. Dittrich, Thomas R. Ward
Published in: Angewandte Chemie International Edition, Issue 14337851, 2022, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202207328