Directed Evolution of Artificial Metalloenzymes for In Vivo Applications

Publicaciones

A Dual Anchoring Strategy for the Directed Evolution of Improved Artificial Transfer Hydrogenases Based on Carbonic Anhydrase.

Autores: Alina Stein; Dongping Chen; Nico V. Igareta; Yoann Cotelle; Johannes G. Rebelein; Thomas R. Ward
Publicado en: ACS Central Science, Edición 23747943, 2021, ISSN 2374-7943
Editor: American Chemical Society
DOI: 10.1021/acscentsci.1c00825

Bioorthogonal strategies for the in vivo synthesis or release of drugs

Autores: Boris Lozhkin, Thomas R.Ward
Publicado en: Bioorganic & Medicinal Chemistry, Edición 09680896, 2021, ISSN 0968-0896
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.bmc.2021.116310

Functional and morphological adaptation in DNA protocells via signal processing prompted by artificial metalloenzymes

Autores: Avik Samanta, Valerio Sabatino, Thomas R. Ward, Andreas Walther
Publicado en: Nature Nanotechnology, Edición 15/11, 2020, Página(s) 914-921, ISSN 1748-3387
Editor: Nature Publishing Group
DOI: 10.1038/s41565-020-0761-y

Enantioselective Hydroxylation of Benzylic C(sp 3 )–H Bonds by an Artificial Iron Hydroxylase Based on the Biotin–Streptavidin Technology

Autores: Joan Serrano-Plana, Corentin Rumo, Johannes G. Rebelein, Ryan L. Peterson, Maxime Barnet, Thomas R. Ward
Publicado en: Journal of the American Chemical Society, Edición 142/24, 2020, Página(s) 10617-10623, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.0c02788

Directed Evolution of a Surface-Displayed Artificial Allylic Deallylase Relying on a GFP Reporter Protein

Autores: Alain Baiyoumy; Jaicy Vallapurackal; Fabian Schwizer; Tillmann Heinisch; Tsvetan Kardashliev; Martin Held; Sven Panke; Thomas R. Ward
Publicado en: ACS Catalysis, Edición 21555435, 2021, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.3929/ethz-b-000506507

An Artificial Metalloenzyme Based on a Copper Heteroscorpionate Enables sp3 C–H Functionalization via Intramolecular Carbene Insertion

Autores: Corentin Rumo, Alina Stein, Juliane Klehr, Ryo Tachibana, Alessandro Prescimone, Daniel Häussinger, Thomas R. Ward
Publicado en: Journal of the American Chemical Society, Edición 00027863, 2022, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.2c03311

Engineering a Metathesis-Catalyzing Artificial Metalloenzyme Based on HaloTag

Autores: Sandro Fischer, Thomas R. Ward, and Alexandria D. Liang
Publicado en: ACS Catalysis, Edición 21555435, 2021, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.1c01470

Droplet Microfluidics and Directed Evolution of Enzymes: an Intertwined Journey

Autores: Ariane Stucki, Jaicy Vallapurackal, Thomas R. Ward, Petra S Dittrich
Publicado en: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202016154

Design and evolution of chimeric streptavidin for protein-enabled dual gold catalysis

Autores: Fadri Christoffel, Nico V. Igareta, Michela M. Pellizzoni, Laura Tiessler-Sala, Boris Lozhkin, Daniel C. Spiess, Agustí Lledós, Jean-Didier Maréchal, Ryan L. Peterson, Thomas R. Ward
Publicado en: Nature Catalysis, Edición 25201158, 2021, ISSN 2520-1158
Editor: Nature Publishing Group
DOI: 10.1038/s41929-021-00651-9

Chemo-enzymatic cascades to produce cycloalkenes from bio-based resources

Autores: Shuke Wu, Yi Zhou, Daniel Gerngross, Markus Jeschek, Thomas R. Ward
Publicado en: Nature Communications, Edición 10/1, 2019, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-019-13071-y

Streptavidin-Enzyme Linked Aggregates for the One-Step Assembly and Purification of Enzyme Cascades

Autores: Hendrik Mallin, Thomas R. Ward
Publicado en: ChemCatChem, Edición 10, 2018, Página(s) 2810 –2816, ISSN 1867-3880
Editor: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.201800162

Photo-Driven Hydrogen Evolution by an Artificial Hydrogenase Utilizing the Biotin-Streptavidin Technology

Autores: Sascha G. Keller, Benjamin Probst, Tillmann Heinisch, Roger Alberto, Thomas R. Ward
Publicado en: Helvetica Chimica Acta, Edición 101/4, 2018, Página(s) e1800036, ISSN 0018-019X
Editor: Verlag Helvetica Chimica Acta
DOI: 10.1002/hlca.201800036

Streptavidin as a Scaffold for Light-Induced Long-Lived Charge Separation

Autores: Sascha G. Keller, Andrea Pannwitz, Hendrik Mallin, Oliver S. Wenger, Thomas R. Ward
Publicado en: Chemistry - A European Journal, Edición 23/71, 2017, Página(s) 18019-18024, ISSN 0947-6539
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201703885

In vivo catalyzed new-to-nature reactions

Autores: Johannes G Rebelein, Thomas R Ward
Publicado en: Current Opinion in Biotechnology, Edición 53, 2018, Página(s) 106-114, ISSN 0958-1669
Editor: Elsevier BV
DOI: 10.1016/j.copbio.2017.12.008

Directed Evolution of an Artificial Imine Reductase

Autores: Martina Hestericová, Tillman Heinisch, Lur Alonso-Cotchico, Jean-Didier Maréchal, Pietro Vidossich, Thomas R. Ward
Publicado en: Angewandte Chemie International Edition, Edición 57/7, 2018, Página(s) 1863-1868, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201711016

A cell-penetrating artificial metalloenzyme regulates a gene switch in a designer mammalian cell

Autores: Yasunori Okamoto, Ryosuke Kojima, Fabian Schwizer, Eline Bartolami, Tillmann Heinisch, Stefan Matile, Martin Fussenegger, Thomas R. Ward
Publicado en: Nature Communications, Edición 9/1, 2018, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-018-04440-0

An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin

Autores: Jingming Zhao, Daniel G. Bachmann, Markus Lenz, Dennis G. Gillingham, Thomas R. Ward
Publicado en: Catalysis Science & Technology, Edición 8/9, 2018, Página(s) 2294-2298, ISSN 2044-4753
Editor: Royal Society of Chemistry
DOI: 10.1039/C8CY00646F

Cross-Regulation of an Artificial Metalloenzyme

Autores: Yasunori Okamoto, Thomas R. Ward
Publicado en: Angewandte Chemie International Edition, Edición 56/34, 2017, Página(s) 10156-10160, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201702181

Ferritin encapsulation of artificial metalloenzymes: engineering a tertiary coordination sphere for an artificial transfer hydrogenase

Autores: Martina Hestericová, Tillmann Heinisch, Markus Lenz, Thomas R. Ward
Publicado en: Dalton Transactions, Edición 47/32, 2018, Página(s) 10837-10841, ISSN 1477-9226
Editor: Royal Society of Chemistry
DOI: 10.1039/c8dt02224k

Genetic Engineering of an Artificial Metalloenzyme for Transfer Hydrogenation of a Self-Immolative Substrate in Escherichia coli’ s Periplasm

Autores: Jingming Zhao, Johannes G. Rebelein, Hendrik Mallin, Christian Trindler, Michela M. Pellizzoni, Thomas R. Ward
Publicado en: Journal of the American Chemical Society, Edición 140/41, 2018, Página(s) 13171-13175, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.8b07189

Chemical Optimization of Whole-Cell Transfer Hydrogenation Using Carbonic Anhydrase as Host Protein

Autores: Johannes G. Rebelein, Yoann Cotelle, Brett Garabedian, Thomas R. Ward
Publicado en: ACS Catalysis, Edición 9/5, 2019, Página(s) 4173-4178, ISSN 2155-5435
Editor: American Chemical Society
DOI: 10.1021/acscatal.9b01006

Artificial Metalloenzymes Based on the Biotin–Streptavidin Technology: Enzymatic Cascades and Directed Evolution

Autores: Alexandria Deliz Liang, Joan Serrano-Plana, Ryan L. Peterson, Thomas R. Ward
Publicado en: Accounts of Chemical Research, Edición 52/3, 2019, Página(s) 585-595, ISSN 0001-4842
Editor: American Chemical Society
DOI: 10.1021/acs.accounts.8b00618

E. coli surface display of streptavidin for directed evolution of an allylic deallylase

Autores: Tillmann Heinisch, Fabian Schwizer, Brett Garabedian, Eszter Csibra, Markus Jeschek, Jaicy Vallapurackal, Vitor B. Pinheiro, Philippe Marlière, Sven Panke, Thomas R. Ward
Publicado en: Chemical Science, Edición 9/24, 2018, Página(s) 5383-5388, ISSN 2041-6520
Editor: Royal Society of Chemistry
DOI: 10.1039/c8sc00484f

Artificial Metalloenzymes: Challenges and Opportunities

Autores: Holly J. Davis, Thomas R. Ward
Publicado en: ACS Central Science, Edición 5/7, 2019, Página(s) 1120-1136, ISSN 2374-7943
Editor: American Chemical Society
DOI: 10.1021/acscentsci.9b00397

“Close-to-Release”: Spontaneous Bioorthogonal Uncaging Resulting from Ring-Closing Metathesis

Autores: Valerio Sabatino, Johannes G. Rebelein, Thomas R. Ward
Publicado en: Journal of the American Chemical Society, Edición 141/43, 2019, Página(s) 17048-17052, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.9b07193

Breaking Symmetry: Engineering Single-Chain Dimeric Streptavidin as Host for Artificial Metalloenzymes

Autores: Shuke Wu, Yi Zhou, Johannes G. Rebelein, Miriam Kuhn, Hendrik Mallin, Jingming Zhao, Nico V. Igareta, Thomas R. Ward
Publicado en: Journal of the American Chemical Society, Edición 141/40, 2019, Página(s) 15869-15878, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.9b06923

Incorporation of metal-chelating unnatural amino acids into halotag for allylic deamination

Autores: Alina Stein, Alexandria Deliz Liang, Reyhan Sahin, Thomas R.Ward
Publicado en: Journal of Organometallic Chemistry, Edición 0022328X, 2022, ISSN 0022-328X
Editor: Elsevier BV
DOI: 10.1016/j.jorganchem.2022.122272

Synthesis of N-Substituted Indoles via Aqueous Ring-Closing Metathesis

Autores: Valerio Sabatino, Dario Staub, Thomas R. Ward
Publicado en: Catalysis Letters, Edición 151/1, 2021, Página(s) 1-7, ISSN 1011-372X
Editor: Kluwer Academic Publishers
DOI: 10.1007/s10562-020-03271-3

Ultrahigh-Throughput Screening of an Artificial Metalloenzyme using Double Emulsions

Autores: Jaicy Vallapurackal, Ariane Stucki, Alexandria Deliz Liang, Juliane Klehr, Petra S. Dittrich, Thomas R. Ward
Publicado en: Angewandte Chemie International Edition, Edición 14337851, 2022, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202207328

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