Directed Evolution of Artificial Metalloenzymes for In Vivo Applications

Project Information

DrEAM

Grant agreement ID: 694424

DOI 10.3030/694424

Project closed

EC signature date 18 August 2016

Start date 1 October 2016

End date 30 September 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 490 700,00

EU contribution € 2 490 700,00

Coordinated by

UNIVERSITAT BASEL
Switzerland

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (29)

A Dual Anchoring Strategy for the Directed Evolution of Improved Artificial Transfer Hydrogenases Based on Carbonic Anhydrase.

Author(s): Alina Stein; Dongping Chen; Nico V. Igareta; Yoann Cotelle; Johannes G. Rebelein; Thomas R. Ward
Published in: ACS Central Science, Issue 23747943, 2021, ISSN 2374-7943
Publisher: American Chemical Society
DOI: 10.1021/acscentsci.1c00825

Bioorthogonal strategies for the in vivo synthesis or release of drugs

Author(s): Boris Lozhkin, Thomas R.Ward
Published in: Bioorganic & Medicinal Chemistry, Issue 09680896, 2021, ISSN 0968-0896
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.bmc.2021.116310

Functional and morphological adaptation in DNA protocells via signal processing prompted by artificial metalloenzymes

Author(s): Avik Samanta, Valerio Sabatino, Thomas R. Ward, Andreas Walther
Published in: Nature Nanotechnology, Issue 15/11, 2020, Page(s) 914-921, ISSN 1748-3387
Publisher: Nature Publishing Group
DOI: 10.1038/s41565-020-0761-y

Enantioselective Hydroxylation of Benzylic C(sp 3 )–H Bonds by an Artificial Iron Hydroxylase Based on the Biotin–Streptavidin Technology

Author(s): Joan Serrano-Plana, Corentin Rumo, Johannes G. Rebelein, Ryan L. Peterson, Maxime Barnet, Thomas R. Ward
Published in: Journal of the American Chemical Society, Issue 142/24, 2020, Page(s) 10617-10623, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c02788

Directed Evolution of a Surface-Displayed Artificial Allylic Deallylase Relying on a GFP Reporter Protein

Author(s): Alain Baiyoumy; Jaicy Vallapurackal; Fabian Schwizer; Tillmann Heinisch; Tsvetan Kardashliev; Martin Held; Sven Panke; Thomas R. Ward
Published in: ACS Catalysis, Issue 21555435, 2021, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.3929/ethz-b-000506507

An Artificial Metalloenzyme Based on a Copper Heteroscorpionate Enables sp3 C–H Functionalization via Intramolecular Carbene Insertion

Author(s): Corentin Rumo, Alina Stein, Juliane Klehr, Ryo Tachibana, Alessandro Prescimone, Daniel Häussinger, Thomas R. Ward
Published in: Journal of the American Chemical Society, Issue 00027863, 2022, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.2c03311

Engineering a Metathesis-Catalyzing Artificial Metalloenzyme Based on HaloTag

Author(s): Sandro Fischer, Thomas R. Ward, and Alexandria D. Liang
Published in: ACS Catalysis, Issue 21555435, 2021, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.1c01470

Droplet Microfluidics and Directed Evolution of Enzymes: an Intertwined Journey

Author(s): Ariane Stucki, Jaicy Vallapurackal, Thomas R. Ward, Petra S Dittrich
Published in: Angewandte Chemie International Edition, 2021, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202016154

Design and evolution of chimeric streptavidin for protein-enabled dual gold catalysis

Author(s): Fadri Christoffel, Nico V. Igareta, Michela M. Pellizzoni, Laura Tiessler-Sala, Boris Lozhkin, Daniel C. Spiess, Agustí Lledós, Jean-Didier Maréchal, Ryan L. Peterson, Thomas R. Ward
Published in: Nature Catalysis, Issue 25201158, 2021, ISSN 2520-1158
Publisher: Nature Publishing Group
DOI: 10.1038/s41929-021-00651-9

Chemo-enzymatic cascades to produce cycloalkenes from bio-based resources

Author(s): Shuke Wu, Yi Zhou, Daniel Gerngross, Markus Jeschek, Thomas R. Ward
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-13071-y

Streptavidin-Enzyme Linked Aggregates for the One-Step Assembly and Purification of Enzyme Cascades

Author(s): Hendrik Mallin, Thomas R. Ward
Published in: ChemCatChem, Issue 10, 2018, Page(s) 2810 –2816, ISSN 1867-3880
Publisher: Wiley - VCH Verlag GmbH & CO. KGaA
DOI: 10.1002/cctc.201800162

Photo-Driven Hydrogen Evolution by an Artificial Hydrogenase Utilizing the Biotin-Streptavidin Technology

Author(s): Sascha G. Keller, Benjamin Probst, Tillmann Heinisch, Roger Alberto, Thomas R. Ward
Published in: Helvetica Chimica Acta, Issue 101/4, 2018, Page(s) e1800036, ISSN 0018-019X
Publisher: Verlag Helvetica Chimica Acta
DOI: 10.1002/hlca.201800036

Streptavidin as a Scaffold for Light-Induced Long-Lived Charge Separation

Author(s): Sascha G. Keller, Andrea Pannwitz, Hendrik Mallin, Oliver S. Wenger, Thomas R. Ward
Published in: Chemistry - A European Journal, Issue 23/71, 2017, Page(s) 18019-18024, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201703885

In vivo catalyzed new-to-nature reactions

Author(s): Johannes G Rebelein, Thomas R Ward
Published in: Current Opinion in Biotechnology, Issue 53, 2018, Page(s) 106-114, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2017.12.008

Directed Evolution of an Artificial Imine Reductase

Author(s): Martina Hestericová, Tillman Heinisch, Lur Alonso-Cotchico, Jean-Didier Maréchal, Pietro Vidossich, Thomas R. Ward
Published in: Angewandte Chemie International Edition, Issue 57/7, 2018, Page(s) 1863-1868, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201711016

A cell-penetrating artificial metalloenzyme regulates a gene switch in a designer mammalian cell

Author(s): Yasunori Okamoto, Ryosuke Kojima, Fabian Schwizer, Eline Bartolami, Tillmann Heinisch, Stefan Matile, Martin Fussenegger, Thomas R. Ward
Published in: Nature Communications, Issue 9/1, 2018, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-04440-0

An artificial metalloenzyme for carbene transfer based on a biotinylated dirhodium anchored within streptavidin

Author(s): Jingming Zhao, Daniel G. Bachmann, Markus Lenz, Dennis G. Gillingham, Thomas R. Ward
Published in: Catalysis Science & Technology, Issue 8/9, 2018, Page(s) 2294-2298, ISSN 2044-4753
Publisher: Royal Society of Chemistry
DOI: 10.1039/C8CY00646F

Cross-Regulation of an Artificial Metalloenzyme

Author(s): Yasunori Okamoto, Thomas R. Ward
Published in: Angewandte Chemie International Edition, Issue 56/34, 2017, Page(s) 10156-10160, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201702181

Ferritin encapsulation of artificial metalloenzymes: engineering a tertiary coordination sphere for an artificial transfer hydrogenase

Author(s): Martina Hestericová, Tillmann Heinisch, Markus Lenz, Thomas R. Ward
Published in: Dalton Transactions, Issue 47/32, 2018, Page(s) 10837-10841, ISSN 1477-9226
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8dt02224k

Genetic Engineering of an Artificial Metalloenzyme for Transfer Hydrogenation of a Self-Immolative Substrate in Escherichia coli’ s Periplasm

Author(s): Jingming Zhao, Johannes G. Rebelein, Hendrik Mallin, Christian Trindler, Michela M. Pellizzoni, Thomas R. Ward
Published in: Journal of the American Chemical Society, Issue 140/41, 2018, Page(s) 13171-13175, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.8b07189

Chemical Optimization of Whole-Cell Transfer Hydrogenation Using Carbonic Anhydrase as Host Protein

Author(s): Johannes G. Rebelein, Yoann Cotelle, Brett Garabedian, Thomas R. Ward
Published in: ACS Catalysis, Issue 9/5, 2019, Page(s) 4173-4178, ISSN 2155-5435
Publisher: American Chemical Society
DOI: 10.1021/acscatal.9b01006

Artificial Metalloenzymes Based on the Biotin–Streptavidin Technology: Enzymatic Cascades and Directed Evolution

Author(s): Alexandria Deliz Liang, Joan Serrano-Plana, Ryan L. Peterson, Thomas R. Ward
Published in: Accounts of Chemical Research, Issue 52/3, 2019, Page(s) 585-595, ISSN 0001-4842
Publisher: American Chemical Society
DOI: 10.1021/acs.accounts.8b00618

E. coli surface display of streptavidin for directed evolution of an allylic deallylase

Author(s): Tillmann Heinisch, Fabian Schwizer, Brett Garabedian, Eszter Csibra, Markus Jeschek, Jaicy Vallapurackal, Vitor B. Pinheiro, Philippe Marlière, Sven Panke, Thomas R. Ward
Published in: Chemical Science, Issue 9/24, 2018, Page(s) 5383-5388, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8sc00484f

Artificial Metalloenzymes: Challenges and Opportunities

Author(s): Holly J. Davis, Thomas R. Ward
Published in: ACS Central Science, Issue 5/7, 2019, Page(s) 1120-1136, ISSN 2374-7943
Publisher: American Chemical Society
DOI: 10.1021/acscentsci.9b00397

“Close-to-Release”: Spontaneous Bioorthogonal Uncaging Resulting from Ring-Closing Metathesis

Author(s): Valerio Sabatino, Johannes G. Rebelein, Thomas R. Ward
Published in: Journal of the American Chemical Society, Issue 141/43, 2019, Page(s) 17048-17052, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.9b07193

Breaking Symmetry: Engineering Single-Chain Dimeric Streptavidin as Host for Artificial Metalloenzymes

Author(s): Shuke Wu, Yi Zhou, Johannes G. Rebelein, Miriam Kuhn, Hendrik Mallin, Jingming Zhao, Nico V. Igareta, Thomas R. Ward
Published in: Journal of the American Chemical Society, Issue 141/40, 2019, Page(s) 15869-15878, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.9b06923

Incorporation of metal-chelating unnatural amino acids into halotag for allylic deamination

Author(s): Alina Stein, Alexandria Deliz Liang, Reyhan Sahin, Thomas R.Ward
Published in: Journal of Organometallic Chemistry, Issue 0022328X, 2022, ISSN 0022-328X
Publisher: Elsevier BV
DOI: 10.1016/j.jorganchem.2022.122272

Synthesis of N-Substituted Indoles via Aqueous Ring-Closing Metathesis

Author(s): Valerio Sabatino, Dario Staub, Thomas R. Ward
Published in: Catalysis Letters, Issue 151/1, 2021, Page(s) 1-7, ISSN 1011-372X
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s10562-020-03271-3

Ultrahigh-Throughput Screening of an Artificial Metalloenzyme using Double Emulsions

Author(s): Jaicy Vallapurackal, Ariane Stucki, Alexandria Deliz Liang, Juliane Klehr, Petra S. Dittrich, Thomas R. Ward
Published in: Angewandte Chemie International Edition, Issue 14337851, 2022, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202207328