Nanoscale live-cell activity sensing using smart probes and imaging

Pubblicazioni

Structure–Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System**

Autori: Elke De Zitter, Siewert Hugelier, Sam Duwé, Wim Vandenberg, Alison G. Tebo, Luc Van Meervelt, Peter Dedecker
Pubblicato in: Angewandte Chemie International Edition, Numero 60/18, 2021, Pagina/e 10073-10081, ISSN 1433-7851
Editore: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202015201

Design of experiments for the optimization of SOFI super-resolution microscopy imaging

Autori: Dario Cevoli, Raffaele Vitale, Wim Vandenberg, Siewert Hugelier, Robin Van den Eynde, Peter Dedecker, Cyril Ruckebusch
Pubblicato in: Biomedical Optics Express, Numero 12/5, 2021, Pagina/e 2617, ISSN 2156-7085
Editore: The Optical Society
DOI: 10.1364/boe.421168

Fluorophore unmixing based on bleaching and recovery kinetics using MCR-ALS.

Autori: Siewert Hugelier; R Van den Eynde; Wim Vandenberg; Wim Vandenberg; Peter Dedecker
Pubblicato in: Talanta, Numero 2, 2021, ISSN 1873-3573
Editore: Elsevier
DOI: 10.1016/j.talanta.2021.122117

Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification

Autori: Franziska Bierbuesse,Anaïs C Bourges,Vincent Gielen,Viola Mönkemöller,Wim Vandenberg,Yi Shen,Johan Hofkens,Pieter Vanden Berghe,Robert E Campbell,Benjamien Moeyaert,Peter Dedecker
Pubblicato in: Nature Communications, 2022, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-022-29508-w

Spectrally Tunable Förster Resonance Energy Transfer-Based Biosensors Using Organic Dye Grafting

Autori: Marco Dalla Vecchia; Amandine Conte-Daban; Benjamin Cappe; Wim Vandenberg; Peter Vandenabeele; Franck B. Riquet; Peter Dedecker
Pubblicato in: ACS Sensors, Numero 3, 2022, ISSN 2379-3694
Editore: ACS
DOI: 10.1021/acssensors.2c00066

Self-contained and modular structured illumination microscope

Autori: Robin Van den Eynde, Wim Vandenberg, Siewert Hugelier, Arno Bouwens, Johan Hofkens, Marcel Müller, Peter Dedecker
Pubblicato in: Biomedical Optics Express, Numero 12/7, 2021, Pagina/e 4414, ISSN 2156-7085
Editore: The Optical Society
DOI: 10.1364/boe.423492

Orthogonal fluorescent chemogenetic reporters for multicolor imaging

Autori: Alison G. Tebo; Benjamien Moeyaert; Marion Thauvin; Marion Thauvin; Irene Carlon-Andres; Dorothea Böken; Michel Volovitch; Michel Volovitch; Sergi Padilla-Parra; Sergi Padilla-Parra; Peter Dedecker; Sophie Vriz; Sophie Vriz; Arnaud Gautier; Arnaud Gautier
Pubblicato in: ISSN: 1552-4450, Numero 15524450, 2020, ISSN 1552-4450
Editore: Nature Publishing Group
DOI: 10.1101/2020.04.04.022111

Model-free pixelation correction in SOFI imaging

Autori: Franziska Bierbuesse; Vincent Gielen; Wim Vandenberg; Peter Dedecker
Pubblicato in: OSA Continuum, Numero 25787519, 2021, ISSN 2578-7519
Editore: OSA
DOI: 10.1364/osac.413107

Super-resolution imaging goes fast and deep

Autori: Sam Duwé, Peter Dedecker
Pubblicato in: Nature Methods, Numero 14/11, 2017, Pagina/e 1042-1044, ISSN 1548-7091
Editore: Nature Publishing Group
DOI: 10.1038/nmeth.4484

Correcting for photodestruction in super-resolution optical fluctuation imaging

Autori: Yves Peeters, Wim Vandenberg, Sam Duwé, Arno Bouwens, Tomáš Lukeš, Cyril Ruckebusch, Theo Lasser, Peter Dedecker
Pubblicato in: Scientific Reports, Numero 7/1, 2017, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-017-09666-4

Reduced Fluorescent Protein Switching Fatigue by Binding-Induced Emissive State Stabilization

Autori: Thijs Roebroek, Sam Duwé, Wim Vandenberg, Peter Dedecker
Pubblicato in: International Journal of Molecular Sciences, Numero 18/9, 2017, Pagina/e 2015, ISSN 1422-0067
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms18092015

Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

Autori: S. Duwé, W. Vandenberg, P. Dedecker
Pubblicato in: Chemical Communications, Numero 53/53, 2017, Pagina/e 7242-7245, ISSN 1359-7345
Editore: Royal Society of Chemistry
DOI: 10.1039/c7cc02344h

Efficient switching of mCherry fluorescence using chemical caging

Autori: Bas M. C. Cloin, Elke De Zitter, Desiree Salas, Vincent Gielen, Gert E. Folkers, Marina Mikhaylova, Maike Bergeler, Bartosz Krajnik, Jeremy Harvey, Casper C. Hoogenraad, Luc Van Meervelt, Peter Dedecker, Lukas C. Kapitein
Pubblicato in: Proceedings of the National Academy of Sciences, Numero 114/27, 2017, Pagina/e 7013-7018, ISSN 0027-8424
Editore: National Academy of Sciences
DOI: 10.1073/pnas.1617280114

Effect of probe diffusion on the SOFI imaging accuracy

Autori: Wim Vandenberg, Peter Dedecker
Pubblicato in: Scientific Reports, Numero 7/1, 2017, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/srep44665

An extended quantitative model for super-resolution optical fluctuation imaging (SOFI)

Autori: Wim Vandenberg, Marcel Leutenegger, Sam Duwé, Peter Dedecker
Pubblicato in: Optics Express, Numero 27/18, 2019, Pagina/e 25749, ISSN 1094-4087
Editore: Optical Society of America
DOI: 10.1364/oe.27.025749

Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM

Autori: Elke De Zitter, Daniel Thédié, Viola Mönkemöller, Siewert Hugelier, Joël Beaudouin, Virgile Adam, Martin Byrdin, Luc Van Meervelt, Peter Dedecker, Dominique Bourgeois
Pubblicato in: Nature Methods, Numero 16/8, 2019, Pagina/e 707-710, ISSN 1548-7091
Editore: Nature Publishing Group
DOI: 10.1038/s41592-019-0462-3

Spatio-temporal correlation super-resolution optical fluctuation imaging

Autori: A. Purohit, W. Vandenberg, T. Dertinger, D. Wöll, P. Dedecker, J. Enderlein
Pubblicato in: EPL (Europhysics Letters), Numero 125/2, 2019, Pagina/e 20005, ISSN 1286-4854
Editore: IOP Publishing
DOI: 10.1209/0295-5075/125/20005

Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI)

Autori: Robin Van den Eynde, Alice Sandmeyer, Wim Vandenberg, Sam Duwé, Wolfgang Hübner, Thomas Huser, Peter Dedecker, Marcel Müller
Pubblicato in: Journal of Physics: Photonics, Numero 1/4, 2019, Pagina/e 044001, ISSN 2515-7647
Editore: IOP Science
DOI: 10.1088/2515-7647/ab36ae

Optimizing the fluorescent protein toolbox and its use

Autori: Sam Duwé, Peter Dedecker
Pubblicato in: Current Opinion in Biotechnology, Numero 58, 2019, Pagina/e 183-191, ISSN 0958-1669
Editore: Elsevier BV
DOI: 10.1016/j.copbio.2019.04.006

Presenting a codon-optimized palette of fluorescent proteins for use in Candida albicans

Autori: Wouter Van Genechten, Liesbeth Demuyser, Peter Dedecker, Patrick Van Dijck
Pubblicato in: Scientific Reports, Numero 10/1, 2020, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-020-63308-w

SOFIevaluator: a strategy for the quantitative quality assessment of SOFI data

Autori: Benjamien Moeyaert, Wim Vandenberg, Peter Dedecker
Pubblicato in: Biomedical Optics Express, Numero 11/2, 2020, Pagina/e 636, ISSN 2156-7085
Editore: The Optical Society
DOI: 10.1364/boe.382278

Quantitative Model for Reversibly Photoswitchable Sensors

Autori: Adelizzi, Beatrice; Gielen, Vincent; Le Saux, Thomas; Dedecker, Peter; Jullien, Ludovic
Pubblicato in: "ACS Sensors, American Chemical Society, 2021, ⟨10.1021/acssensors.0c02414⟩", Numero 3, 2021, ISSN 2379-3694
Editore: ACS

Smoothness correction for better SOFI imaging.

Autori: Siewert Hugelier; Wim Vandenberg; Tomas Lukes; Kristin S. Grußmayer; Kristin S. Grußmayer; Paul H. C. Eilers; Peter Dedecker; Cyril Ruckebusch
Pubblicato in: Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021), Numero 11, 2021, Pagina/e 1, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-021-87164-4

Quantitative Model for Reversibly Photoswitchable Sensors.

Autori: Beatrice Adelizzi; Vincent Gielen; Thomas Le Saux; Peter Dedecker; Ludovic Jullien
Pubblicato in: ACS Sensors, Numero 23793694, 2021, ISSN 2379-3694
Editore: American Chemical Society
DOI: 10.1021/acssensors.0c02414

Simultaneous readout of multiple FRET pairs using photochromism

Autori: Thijs Roebroek, Wim Vandenberg, François Sipieter, Siewert Hugelier, Christophe Stove, Jin Zhang, Peter Dedecker
Pubblicato in: Nature Communications, Numero 12/1, 2021, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-021-22043-0

Separation of spectrally overlapping fluorophores using intra-exposure excitation modulation

Autori: Hana Valenta; Siewert Hugelier; Sam Duwé; Giulia Lo Gerfo; Marcel Müller; Peter Dedecker; Wim Vandenberg
Pubblicato in: Biophysical Reports, Numero 26670747, 2021, ISSN 2667-0747
Editore: Cell Press
DOI: 10.1016/j.bpr.2021.100026

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