Objetivo Implementing highly parallel cell based assays able to replace animal testing is crucial for a large variety of industries. Some cell-based assays have already been achieved using multi-wells plastic plates where individual cell cultures and their stimulations are realised in wells. Unfortunately these novel technologies did not allow to get the level of discoveries that was expected when phenotyping cells for drug discovery and toxicology. DNA chips experiments have already permit massive analyses of animal and human genomes particularly used for in vitro toxicological studies. More information could be obtained replacing the DNA spots by drops of cell culture allowing to establish the relationship between cell phenotypes and chemical toxicity. The aim of this two year ToxDrop project is to develop a Cell on Chip technology where various phenotypes illustrating cytotoxicity can be characterised through the phenotyping of cells in highly parallel cell based reactions on a single chip. The substrate of the biochip will allow to achieve the necessary precision in the formation of the cellular nanodrops and the high content analyses of cell cultures based on fluorescent, high contrast optical based detection as well as mass spectrometry phenotypic signatures. The project will help three European SMEs to get access to the pioneer technology of the Cell on Chip which could be used in particular for cell based in vitro toxicological studies by the industry. The ToxDrop validation will be planned through discussions with ECVAM and realised through tests in cellular nanodrops by academic laboratories specialised in pharmacological and chemical toxicological studies. In order to reproduce on the Cell on Chip several assays previously tested in animals, toxicity will be carried out in nanodrops containing several types of cells issued from various organs of several species. In particular some problems never solved without the use of animal will be tackled... Ámbito científico medical and health sciencesbasic medicinepharmacology and pharmacydrug discoverynatural sciencesbiological sciencesgeneticsDNAnatural sciencesphysical sciencesopticsmicroscopynatural scienceschemical sciencesanalytical chemistrymass spectrometrymedical and health sciencesbasic medicinetoxicology Palabras clave Cell On chip Nanotechnology cell based phenotyping cytotoxicity gene transfection microarray non-animal testing siRNA Programa(s) FP6-POLICIES - Policy support: Specific activities covering wider field of research under the Focusing and Integrating Community Research programme 2002-2006. Tema(s) POLICIES-2.2 - Public health issues, including epidemiology contributing to disease prevention and responses to emerging rare and communicable diseases, allergies, procedures for secure blood and organ donations, non-animal test methods Convocatoria de propuestas FP6-2003-SSP-3 Consulte otros proyectos de esta convocatoria Régimen de financiación STIP - Specific Targeted Innovation Project Coordinador COMMISSARIAT À L'ENERGIE ATOMIQUE Aportación de la UE Sin datos Dirección 17 rue des martyrs cedex9 GRENOBLE Francia Ver en el mapa Coste total Sin datos Participantes (7) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE Francia Aportación de la UE Sin datos Dirección 101 rue de Tolbiac PARIS Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos UNIVERSITÉ CLAUDE BERNARD LYON 1 Francia Aportación de la UE Sin datos Dirección 43 Boulevard du 11 novembre 1918 VILLEURBANNE Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos ECOLE POLYTECHNIQUE FÉDÉRALE DE LAUSANNE Suiza Aportación de la UE Sin datos Dirección EPFL LAUSANNE Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos IMSTAR Francia Aportación de la UE Sin datos Dirección RUE NOTRE DAME DES CHAMPS, 60 PARIS Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos TASCON GESELLSCHAFT FÜR OBERFLÄCHEN- UND MATERIALCHARAKTERISIERUNG MBH Alemania Aportación de la UE Sin datos Dirección Mendelstrasse 11 MÜNSTER Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos VITAMIB SARL Francia Aportación de la UE Sin datos Dirección 29 BOULEVARD DES ALPES MEYLAN CEDEX Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos JOINT RESEARCH CENTRE (JRC) Bélgica Aportación de la UE Sin datos Dirección RUE DE LA LOI 200 BRUSSELS Ver en el mapa Enlaces Sitio web Opens in new window Coste total Sin datos
