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EMBO course on microRNA profiling, Heidelberg, Germany

The European Molecular Biology Organisation (EMBO) will offer a course on 'MicroRNA profiling: from in-situ hybridisation to new-generation sequencing' from 18 to 24 April 2009 in Heidelberg, Germany.

The course will start with a theoretical introduction to microRNA profiling...

18 April 2009 - 18 April 2009
Germany

The European Molecular Biology Organisation (EMBO) will offer a course on 'MicroRNA profiling: from in-situ hybridisation to new-generation sequencing' from 18 to 24 April 2009 in Heidelberg, Germany.

The course will start with a theoretical introduction to microRNA profiling. This will be followed by a combination of hands-on laboratory and bioinformatics work with lectures and tutorials on the following topics:
- quality assessment and quantification of total RNA;
- set-up of the complete microarray-microRNA profiling and in-situ hybridisation experiments;
- setup of a complete quantitative, real time polymerase chain reaction (qPCR) experiment on various systems, reverse transcription and qPCR steps: synthesis of complementary DNA followed by microRNA specific amplification;
- set-up of the complete bead-based microRNA profiling assay;
- data analysis by different algorithms, normalisation approaches;
- correlation of microRNA expression data originating from various methods;
- troubleshooting of all individual steps;
- approaches to microRNA profiling (new-gen sequencing, bead-based, microarray, qPCR, Northern blotting, in-situ hybridisation);
- sample preparation, experimental design/setup, equipment/instrumentation options and quality control for total RNA;
- design of qPCR assays for determination of microRNA expression;
- design of the microarray microRNA experiment;
- data analysis, normalisation and interpretation;
- correlation of transcriptome profiling with microRNA profiling data;
- correlation between various microRNA profiling approaches;
- prediction of microRNA targets and their experimental confirmation.For further information, please visit:
http://www-db.embl.de/jss/EmblGroupsOrg/conf_117#registration