Cost-effective sequencing of full viral genomes has been established. This is a pioneering result which will allow a more in-depth analysis of virus evolution and spread in natural and alternative hosts as well as in crop plants with various levels of resistance to better characterize the impact of virus resistance or susceptibility on virus evolution. New sources of full or partial virus resistances were identified and characterized. This will help building novel combination of natural resistances. Several natural molecules with activities against the virus vectors were identified to develop alternative methods for pest control and reduction of conventional pesticides. Updated procedures for hygiene protocols are also opening new perspectives to mitigate virus outbreaks. Multi-actor tools and guidelines are being implemented to foster the bottom-up approach of the scientific impacts. WP2-4 activities are being integrated in WP5 on IPM to provide growers and the value chain with an integrated strategy for virus and vector management, and a toolbox to help eradicating viruses after infection, with focus on soil, substrates and plant materials. The screening activities will be complemented with molecular analysis to elucidate the regulatory pathways involved in plant responses to viral infections. This will help identify factors involved in the transmission and disease symptom development. It includes understanding the effects of climate change conditions by analysing plant transcription profiles, the role of seed-borne virus infection in disease spread and ways to control it. In-depth characterisation of natural plant responses to viruses and vectors will also contribute to new approaches for rapid screening of germplasms. The focus on new biorational whitefly control strategies will also help implement multiprone approaches to mitigate virus outbreaks. A cross-protection strategy concept will be proven to control ToBRFV. The identification and characterization of natural virus resistance and the development and cultivation of ToBRFV-resistant tomato varieties and ToLCNDV-resistant tomato & cucurbit varieties will provide a long-term solution. VIRTIGATION aims to reduce losses due to diseases caused by ToLCNDV and ToBRFV in tomato and cucurbit by at least 80%, while pesticide usage to control vector population and transmission could be at least halved. The identification of natural molecules for biopesticide development will help reduce insecticide application and dosage, thereby improving the environment for growers and the society. Similarly, screening of the tomato and cucurbit germplasm and a better understanding of crop responses to the two viruses will identify novel sources of natural resistance that will be a basis for implementing IPM measures. VIRTIGATION’s diagnostics and the analysis of virus diversity combined with the online tool for sequencing data upload and analysis will provide details about virus diversity and movement across EU countries that can be used for other emerging viral diseases. Viral pandemics could be prevented by early detection of hyper-virulent isolates, thus limiting the occurrence of plant viral pandemics in vegetable and other crops. VIRTIGATION will contribute to other important pathosystems by developing virus analysis tools, and testing and implementing IPM approaches. Field assessment of IPM practices and exchange of best practices between participating countries will lead to a roadmap to reduce pesticide usage. As environmental conditions can impact vector and virus spread, the project will also develop preventive measures against emerging begomovirus and tobamovirus diseases in countries at risk of pandemics.