HARNESSING THE POWER OF NATURE THROUGH PRODUCTIVE MICROBIAL CONSORTIA IN BIOTECHNOLOGY – MEASURE, MODEL, MASTER

Información del proyecto

PROMICON

Identificador del acuerdo de subvención: 101000733

DOI

10.3030/101000733

Proyecto cerrado

Fecha de la firma de la CE 3 Mayo 2021

Fecha de inicio 1 Junio 2021

Fecha de finalización 31 Mayo 2025

Financiado con arreglo a

• SOCIETAL CHALLENGES - Food security, sustainable agriculture and forestry, marine, maritime and inland water research, and the bioeconomy

Coste total

€ 5 999 712,50

Aportación de la UE

€ 5 999 712,50

Coordinado por

HELMHOLTZ-ZENTRUM FUR UMWELTFORSCHUNG GMBH - UFZ
Germany

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Documents, reports (27)

Selection and characterization of microbiomes under ecological stress
Running on-line single cell analysis platform

Running online single cell analysis platform

A set of optimized P. putida strains overproducing PHACOS from sucrose and 6-ATH.
Project Collection of OMICS data in the Research Ideas and Outcomes journal

Project collection of papers and datasets published in Research Ideas and Outcomes Journal

A set of in silico optimized synthetic microbial consortia overproducing PHA, butanol and H2 from CO2 and sunlight.
Report about Multispectral characterization of microbiome: PHA, EPS, biomass and phycobiliproteins
Rationally engineered 3D Synechococcus/P. putida PHA-producing consortium.
PROMICON Economic Analysis

Report on PROMICON Economic Analysis -intermediate (m24) and final (m48)

SOPs for the operation of the capillary biofilm reactor available for the PROMICON consortium
Quantitative MS-based metabolomics dataset

Quantitative MSbased metabolomics dataset

Future recommendations factsheets based on a detailed report and evaluation of dissemination and communication activities
Modelling of chemotrophs and cyanobacterial-rich microbiomes at (meta)genome-scale.
PROMICON LCA
Report on the phototrophs and chemotrophs optimal conditions for PPP, EPS and PHA production based on WP1 models
Connecting on-line single cell data with automatic evaluation pipelines

Connecting online single cell data with automatic evaluation pipelines

Sucrose overproducer Synechococcus strain based on deletion of competing pathways and population homogenization.
Report and user manual of AI models for measurement of M1.3 parameters from spectral images
A set of optimized P. taiwanensis VLB 120 balancer strains with enhanced c-di-GMP levels.

A set of optimized P taiwanensis VLB 120 balancer strains with enhanced cdiGMP levels

NMR-environomics dataset

NMRenvironomics dataset

Rationally engineered consortium for continuous photofermentative H2 production.
SOPs for the operation of the dual chamber system available for the PROMICON consortium
Meta-functional-environomics reconstruction of natural microbiome

Metafunctionalenvironomics reconstruction of natural microbiome

Characterization of microbial consortia from natural environments
PROMICON S-LCA
A P. putida strain suitable as labor module including sucrose metabolism and lacking negative traits.

A P putida strain suitable as labor module including sucrose metabolism and lacking negative traits

Hybrid AI/physical controller of natural microbiomes
In situ analysis of the biofilm microbiome growing in a CBR accomplished

Websites, patent fillings, videos etc. (1)

Project branding and communication channels set-up and running (logo, promotional materials, website, templates, social media)

Project branding and communication channels setup and running logo promotional materials website templates social media

Demonstrators, pilots, prototypes (1)

Proof of concept shown for operating a dual chamber membrane reactor for the production of PHACOS

Publicaciones

Peer reviewed articles (30)

Development of an Automated Online Flow Cytometry Method to Quantify Cell Density and Fingerprint Bacterial Communities

Autores: Juan López-Gálvez, Konstanze Schiessl, Michael D. Besmer, Carmen Bruckmann, Hauke Harms, Susann Müller
Publicado en: Cells, Edición 12, 2023, Página(s) 1559, ISSN 2073-4409
Editor: MDPI
DOI: 10.3390/cells12121559

New strategy for bioplastic and exopolysaccharides production: Enrichment of field microbiomes with cyanobacteria

Autores: Beatriz Altamira-Algarra, Estel Rueda, Artai Lage, David San León, Juan F. Martínez-Blanch, Juan Nogales, Joan García, Eva Gonzalez-Flo
Publicado en: New Biotechnology, Edición 78, 2023, Página(s) 141-149, ISSN 1871-6784
Editor: Elsevier BV
DOI: 10.1016/j.nbt.2023.10.008

Enhancing pigment production with cyanobacteria-rich microbiomes: Effect of light quality and exposure time

Autores: Marta Bellver, Beatriz Altamira-Algarra, Joan García, Ivet Ferrer, Eva Gonzalez-Flo
Publicado en: Algal Research, Edición 83, 2024, Página(s) 103726, ISSN 2211-9264
Editor: Elsevier BV
DOI: 10.1016/j.algal.2024.103726

Monitoring PHB production in Synechocystis sp. with hyperspectral images

Autores: Francisco Rodríguez Lorenzo; Miguel Placer Lorenzo; Luz Herrero Castilla; Juan Antonio Álvarez Rodríguez; Sandra Iglesias; Santiago Gómez; Juan Manuel Fernández Montenegro; Estel Rueda; Rubén Diez-Montero; Joan Garcia; Eva Gonzalez-Flo
Publicado en: Water Science & Technology, 2022, ISSN 1996-9732
Editor: IWA Publishing
DOI: 10.2166/wst.2022.194

Bioplastic production by harnessing cyanobacteria-rich microbiomes for long-term synthesis

Autores: Beatriz Altamira-Algarra, Artai Lage, Ana Lucía Meléndez, Marc Arnau, Eva Gonzalez-Flo, Joan García
Publicado en: Science of The Total Environment, Edición 954, 2025, Página(s) 176136, ISSN 0048-9697
Editor: Elsevier BV
DOI: 10.1016/j.scitotenv.2024.176136

Novel approaches to energize microbial biocatalysts

Autores: Gonzalo Durante‐Rodríguez, Manuel Carmona, Eduardo Díaz
Publicado en: Environmental Microbiology, Edición 25, 2023, Página(s) 161-166, ISSN 1462-2912
Editor: Blackwell Publishing Inc.
DOI: 10.1111/1462-2920.16254

Toward merging bottom–up and top–down model-based designing of synthetic microbial communities

Autores: David San León & Juan Nogales
Publicado en: Current Opinion in Microbiology, 2022, ISSN 1369-5274
Editor: Elsevier BV
DOI: 10.1016/j.mib.2022.102169

Photosynthetic species composition determines bioplastics production in microbiomes: Strategy to enrich cyanobacteria PHB-producers

Autores: Beatriz Altamira-Algarra, Artai Lage, Joan García, Eva Gonzalez-Flo
Publicado en: Algal Research, Edición 79, 2024, Página(s) 103469, ISSN 2211-9264
Editor: Elsevier BV
DOI: 10.1016/j.algal.2024.103469

Cyanobacteria microbiomes for bioplastic production: Critical review of key factors and challenges in scaling from laboratory to industry set-ups

Autores: Beatriz Altamira-Algarra, Joan Garcia, Eva Gonzalez-Flo
Publicado en: Bioresource Technology, Edición 422, 2025, Página(s) 132231, ISSN 0960-8524
Editor: Elsevier BV
DOI: 10.1016/j.biortech.2025.132231

A double-staining automated flow cytometry method for real-time monitoring of bacteria in continuous bioreactors

Autores: Juan López-Gálvez, Erik Schönfelder, Hanna Mayer, Konstanze Schiessl, Marisa O. D. Silva, Hauke Harms, Susann Müller
Publicado en: A double-staining automated flow cytometry method for real-time monitoring of bacteria in continuous bioreactors, 2025, ISSN 2692-8205
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2025.03.24.644962

Molecular dynamics of photosynthetic electron flow in a biophotovoltaic system

Autores: Jianqi Yuan, Jens Appel, Kirstin Gutekunst, Bin Lai, Jens Olaf Krömer
Publicado en: Environmental Science and Ecotechnology, Edición 23, 2025, Página(s) 100519, ISSN 2666-4984
Editor: Elsevier BV
DOI: 10.1016/j.ese.2024.100519

Improving PHB production in cyanobacteria: Modeling the optimal light regime for growth

Autores: I. Perez Couñago, J.M. Fernandez Montenegro, S. Iglesias Moreira, F. Rodriguez Lorenzo, M. Placer Lorenzo, P. Villar Sola, E. Pancorbo González, J. Illade Quinteiro, L. Herrero Castilla, J.A. Álvarez Rodríguez, B. Altamira Algarra, E. Gonzalez Flo, J. Garcia, F. Guedes, M. Lopez-Garcia, S. Muiños-Landin
Publicado en: New Biotechnology, Edición 89, 2025, Página(s) 73-81, ISSN 1871-6784
Editor: Elsevier BV
DOI: 10.1016/j.nbt.2025.05.005

Exploring and increased acetate biosynthesis in Synechocystis PCC 6803 through insertion of a heterologous phosphoketolase and overexpressing phosphotransacetylase

Autores: Stamatina Roussou, Minmin Pan, Jens O. Krömer, Peter Lindblad
Publicado en: Metabolic Engineering, Edición 88, 2025, Página(s) 250-260, ISSN 1096-7176
Editor: Academic Press
DOI: 10.1016/j.ymben.2025.01.008

Exploiting synergies between microbial electrochemical technologies and synthetic biology

Autores: Bin Lai, Jens Krömer, Federico Aulenta, Hui Wu, Pablo Ivan Nikel
Publicado en: Microbial Biotechnology, Edición 16, 2023, Página(s) 485-488, ISSN 1751-7915
Editor: Wiley
DOI: 10.1111/1751-7915.14208

Robust strategy for bioplastic production from cyanobacteria-enriched microbiomes: insights from gene expression and population dynamics

Autores: Beatriz Altamira-Algarra, Lin Sun, David San León Granado, Lucía Romero-Morillo, Livia Vurro, Juan Nogales, Eva Gonzalez-Flo, Joan Garcia
Publicado en: Chemical Engineering Journal, Edición 517, 2025, Página(s) 164196, ISSN 1385-8947
Editor: Elsevier BV
DOI: 10.1016/j.cej.2025.164196

Rational Design Assisted by Evolutionary Engineering Allows <scp>(De)Construction</scp> and Optimization of Complex Phenotypes in <scp><i>Pseudomonas putida</i> KT2440</scp>

Autores: Blas Blázquez, Juan Nogales
Publicado en: Microbial Biotechnology, Edición 18, 2025, ISSN 1751-7915
Editor: Wiley
DOI: 10.1111/1751-7915.70132

When microbial biotechnology meets material engineering

Autores: Ana M. Hernández-Arriaga, Cristina Campano, Virginia Rivero-Buceta, M. Auxiliadora Prieto
Publicado en: Microbial Biotechnology, 2022, ISSN 1751-7915
Editor: Willey
DOI: 10.1111/1751-7915.13975

A general deep hybrid model for bioreactor systems: Combining first principles with deep neural networks

Autores: José Pinto, Mykaella Mestre, J.Ramos, Rafael S.Costa, Gerald Striedner, RuiOliveira
Publicado en: Computers & Chemical Engineering, 2022, ISSN 0098-1354
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.compchemeng.2022.107952

Cyanobacterial biofilms: from natural systems to applications

Autores: Mahir Bozan, Hannah Berreth, Pia Lindberg, Katja Bühler
Publicado en: Trends in Biotechnology, 2024, ISSN 0167-7799
Editor: Elsevier BV
DOI: 10.1016/j.tibtech.2024.08.005

Challenges, progress, and future perspectives for cyanobacterial polyhydroxyalkanoate production

Autores: Estel Rueda, Eva Gonzalez-Flo, Soumila Mondal, Karl Forchhammer, Dulce María Arias, Katharina Ludwig, Bernhard Drosg, Ines Fritz, C. Raul Gonzalez-Esquer, Sara Pacheco, Joan García
Publicado en: Reviews in Environmental Science and Bio/Technology, Edición 23, 2024, Página(s) 321-350, ISSN 1569-1705
Editor: Kluwer Academic Publishers
DOI: 10.1007/s11157-024-09689-0

A Coculture of Photoautotrophs and Hydrolytic Heterotrophs Enables Efficient Upcycling of Starch from Wastewater toward Biomass-Derived Products: Synergistic Interactions Impacting Metabolism of the Consortium

Autores: Minmin Pan, Yiru Wang, Jens O. Krömer, Xinyu Zhu, Marie Karen Tracy Hong Lin, Irini Angelidaki
Publicado en: Environmental Science &amp; Technology, Edición 57, 2023, Página(s) 15523-15532, ISSN 0013-936X
Editor: American Chemical Society
DOI: 10.1021/acs.est.3c05321

SBML2HYB: a Python interface for SBML compatible hybrid modeling

Autores: José Pinto, Rafael S Costa, Leonardo Alexandre, João Ramos, Rui Oliveira
Publicado en: Bioinformatics, 2023, ISSN 1367-4803
Editor: Oxford University Press
DOI: 10.1093/bioinformatics/btad044

Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes

Autores: Beatriz Altamira-Algarra, Joan García, Cristiana A.V. Torres, Maria A.M. Reis, Eva Gonzalez-Flo
Publicado en: New Biotechnology, Edición 87, 2025, Página(s) 82-92, ISSN 1871-6784
Editor: Elsevier BV
DOI: 10.1016/j.nbt.2025.02.008

Enhancing biodesulfurization by engineering a synthetic dibenzothiophene mineralization pathway

Autores: Igor Martínez, Magdy El-Said Mohamed, José Luis García, Eduardo Díaz
Publicado en: Frontiers in Microbiology, Edición 13, 2022, ISSN 1664-302X
Editor: Frontiers Media
DOI: 10.3389/fmicb.2022.987084

Engineering a Photoautotrophic Microbial Coculture toward Enhanced Biohydrogen Production

Autores: Minmin Pan, Rodrigo Amarante Colpo, Stamatina Roussou, Chang Ding, Peter Lindblad, Jens O. Krömer
Publicado en: Environmental Science &amp; Technology, Edición 59, 2025, Página(s) 337-348, ISSN 0013-936X
Editor: American Chemical Society
DOI: 10.1021/acs.est.4c08629

Ecological forces dictate microbial community assembly processes in bioreactor systems

Autores: Shuang LiSusann Müller
Publicado en: Current Opinion in Biotechnology, 2023, ISSN 0958-1669
Editor: Elsevier BV
DOI: 10.1016/j.copbio.2023.102917

Current advances in engineering cyanobacteria and their applications for photosynthetic butanol production

Autores: Xufeng Liu, Hao Xie, Stamatina Roussou, Peter Lindblad
Publicado en: Current Opinion in Biotechnology, 2021, ISSN 0958-1669
Editor: Elsevier BV
DOI: 10.1016/j.copbio.2021.07.014

Microalgae harvesting for wastewater treatment and resources recovery: A review

Autores: Etiele Greque de Morais, Igor Carvalho Fontes Sampaio, Eva Gonzalez-Flo, Ivet Ferrer, Enrica Uggetti, Joan García
Publicado en: New Biotechnology, Edición 78, 2023, Página(s) 84-94, ISSN 1871-6784
Editor: Elsevier BV
DOI: 10.1016/j.nbt.2023.10.002

Enhancing tellurite and selenite bioconversions by overexpressing a methyltransferase from <i>Aromatoleum</i> sp. CIB

Autores: Elena Alonso‐Fernandes, Helga Fernández‐Llamosas, Irene Cano, Cristina Serrano‐Pelejero, Laura Castro, Eduardo Díaz, Manuel Carmona
Publicado en: Microbial Biotechnology, Edición 16, 2023, Página(s) 915-930, ISSN 1751-7915
Editor: Willey
DOI: 10.1111/1751-7915.14162

New Insights on Metabolic Features of Bacillus subtilis Based on Multistrain Genome-Scale Metabolic Modeling

Autores: Blas Blázquez, David San León, Antonia Rojas, Marta Tortajada, Juan Nogales
Publicado en: International Journal of Molecular Sciences, Edición 24, 2023, Página(s) 7091, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms24087091