Analysis of “difficult” proteins by microfluidic-based hydrogen/deuterium exchange

Project Information

HDXchip

Grant agreement ID: 101003052

DOI

10.3030/101003052

EC signature date 27 January 2021

Start date 1 September 2021

End date 31 August 2026

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 999 752,00

EU contribution

€ 1 999 752,00

Coordinated by

KOBENHAVNS UNIVERSITET
Denmark

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Publications

Peer reviewed articles (5)

PNGase H + variant from <i>Rudaea cellulosilytica</i> with improved deglycosylation efficiency for rapid analysis of eukaryotic <i>N</i> ‐glycans and hydrogen deuterium exchange mass spectrometry analysis of glycoproteins

Author(s): Rui‐Rui Guo; Tian‐Chan Zhang; Thomas Ole Tandrup Lambert; Ting Wang; Josef Voglmeir; Kasper D. Rand; Li Liu
Published in: Rapid Communications in Mass Spectrometry, 2022, ISSN 1097-0231
Publisher: John Wiley & Sons Ltd
DOI: 10.1002/rcm.9376

Development of a PNGase Rc column for online deglycosylation of complex glycoproteins during HDX-MS

Author(s): Thomas Lambert; Marius Gramlich; Luisa Stutzke; Luke Smith; Dingyu Deng; Philipp D. Kaiser; Ulrich Rothbauer; Justin L.P. Benesch; Cornelia Wagner; Maximiliane Koenig; Petr Pompach; Petr Novak; Anne Zeck; Kasper Rand
Published in: Journal of the American Society for Mass Spectrometry, 2023, Page(s) 2556-2566, ISSN 1044-0305
Publisher: Elsevier BV
DOI: 10.1101/2023.07.28.550801

Hydrogen/Deuterium Exchange Mass Spectrometry with Integrated Electrochemical Reduction and Microchip-Enabled Deglycosylation for Epitope Mapping of Heavily Glycosylated and Disulfide-Bonded Proteins

Author(s): Gerard Comamala, Camilla C. Krogh, Vibe S. Nielsen, Jörg P. Kutter, Josef Voglmeir, and Kasper D. Rand*
Published in: Analytical Chemistry, 2021, Page(s) 16330–16340, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.1c01728

Probing the impact of temperature and substrates on the conformational dynamics of the Neurotransmitter:Sodium symporter LeuT.

Author(s): Dionisie Calugareanu; Ingvar R. Möller; Solveig G. Schmidt; Claus J. Loland; Kasper D. Rand
Published in: Journal of Molecular Biology, 2022, Page(s) 167356, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2021.167356

Conformationally Restricted Glycopeptide Backbone Inhibits Gas-Phase H/D Scrambling between Glycan and Peptide Moieties

Author(s): Christian Code; Danwen Qiu; Ilia A. Solov’yov; Jung-Goo Lee; Hyeon-Cheol Shin; Christopher Roland; Celeste Sagui; Damian Houde; Kasper D. Rand; Thomas J. D. Jørgensen
Published in: Journal of the American Chemical Society, 2023, Page(s) 23925-23938, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.3c04068