Analysis of “difficult” proteins by microfluidic-based hydrogen/deuterium exchange

Project Information

HDXchip

Grant agreement ID: 101003052

DOI

10.3030/101003052

EC signature date 27 January 2021

Start date 1 September 2021

End date 30 June 2027

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost

€ 1 999 752,00

EU contribution

€ 1 999 752,00

Coordinated by

KOBENHAVNS UNIVERSITET
Denmark

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Publications

Peer reviewed articles (8)

PNGase H + variant from <i>Rudaea cellulosilytica</i> with improved deglycosylation efficiency for rapid analysis of eukaryotic <i>N</i> ‐glycans and hydrogen deuterium exchange mass spectrometry analysis of glycoproteins

Author(s): Rui‐Rui Guo; Tian‐Chan Zhang; Thomas Ole Tandrup Lambert; Ting Wang; Josef Voglmeir; Kasper D. Rand; Li Liu
Published in: Rapid Communications in Mass Spectrometry, 2022, ISSN 1097-0231
Publisher: John Wiley & Sons Ltd
DOI: 10.1002/rcm.9376

Development of a PNGase Rc column for online deglycosylation of complex glycoproteins during HDX-MS

Author(s): Thomas Lambert; Marius Gramlich; Luisa Stutzke; Luke Smith; Dingyu Deng; Philipp D. Kaiser; Ulrich Rothbauer; Justin L.P. Benesch; Cornelia Wagner; Maximiliane Koenig; Petr Pompach; Petr Novak; Anne Zeck; Kasper Rand
Published in: Journal of the American Society for Mass Spectrometry, 2023, Page(s) 2556-2566, ISSN 1044-0305
Publisher: Elsevier BV
DOI: 10.1101/2023.07.28.550801

Hydrogen/Deuterium Exchange Mass Spectrometry with Integrated Electrochemical Reduction and Microchip-Enabled Deglycosylation for Epitope Mapping of Heavily Glycosylated and Disulfide-Bonded Proteins

Author(s): Gerard Comamala, Camilla C. Krogh, Vibe S. Nielsen, Jörg P. Kutter, Josef Voglmeir, and Kasper D. Rand*
Published in: Analytical Chemistry, 2021, Page(s) 16330–16340, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.1c01728

Probing the impact of temperature and substrates on the conformational dynamics of the Neurotransmitter:Sodium symporter LeuT.

Author(s): Dionisie Calugareanu; Ingvar R. Möller; Solveig G. Schmidt; Claus J. Loland; Kasper D. Rand
Published in: Journal of Molecular Biology, 2022, Page(s) 167356, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2021.167356

Structure and dynamics of GAD65 in complex with an autoimmune polyendocrine syndrome type 2-associated autoantibody

Author(s): Susanne H. D. Ständer, Cyril F. Reboul, Sarah N. Le, Daniel E. Williams, Peter G. Chandler, Mauricio G. S. Costa, David E. Hoke, John D. T. Jimma, James Fodor, Gustavo Fenalti, Stuart I. Mannering, Benjamin T. Porebski, Peter Schofield, Daniel Christ, Malcolm Buckle, Sheena McGowan, Dominika Elmlund, Kasper D. Rand, Ashley M. Buckle
Published in: Nature Communications, Issue 16, 2025, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-025-57492-4

Development of a Thiol–ene Microfluidic Chip for Hydrogen/Deuterium Exchange Mass Spectrometry (HDX-MS)

Author(s): Anton B. Hansen, Rasmus R. Svejdal, Jordan T. Aerts, Vanessa B. P. Stahlhut, Daria Harmash, Minna Hamidi, Drago Sticker, Jörg P. Kutter, Kasper D. Rand
Published in: Analytical Chemistry, Issue 97, 2025, Page(s) 12503-12512, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.4c06230

Developments in hydrogen/deuterium exchange mass spectrometry at sub-zero temperatures

Author(s): Anton Berg Hansen, Omid Rouhi, Kasper D. Rand
Published in: TrAC Trends in Analytical Chemistry, Issue 194, 2026, Page(s) 118539, ISSN 0165-9936
Publisher: Elsevier BV
DOI: 10.1016/j.trac.2025.118539

Conformationally Restricted Glycopeptide Backbone Inhibits Gas-Phase H/D Scrambling between Glycan and Peptide Moieties

Author(s): Christian Code; Danwen Qiu; Ilia A. Solov’yov; Jung-Goo Lee; Hyeon-Cheol Shin; Christopher Roland; Celeste Sagui; Damian Houde; Kasper D. Rand; Thomas J. D. Jørgensen
Published in: Journal of the American Chemical Society, 2023, Page(s) 23925-23938, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.3c04068