Project description
An advanced genetic strategy for increasing crop yield
Soil microorganisms, including arbuscular mycorrhizal fungi (AMF), one of the most widespread and functionally important symbioses on earth, have enormous potential for promoting sustainable agriculture and food security. However, the mass production of a safe and robust inoculum, produced in vitro at an affordable cost, remains difficult. The EU-funded MycUpscaling project will address this challenge and determine the genes responsible for increasing triacylglycerol (TAG) accumulation in the symbiotic interface and for increasing spore numbers to create high-quality, cost-effective AMF inoculants for application in agroecosystems. The project will include combinatorial lipid metabolic engineering, selection of mycorrhized TAG-accumulating hosts, in vitro and in vivo lipid flux analysis and in vitro spore domestication.
Objective
Major scientific challenges nowadays are to preserve the environment, reduce global warming and grow more food to meet the global demand. Mass-producing the right soil microbiota essential to plant health and yield has the potential to be a key part of the next big revolution in the development of sustainable agriculture and food security. Arbuscular mycorrhizal fungi (AMF) are among the most ancient, widespread and functionally important symbioses on Earth that help feed the world. Yet, mass-production of clean (i.e. in vitro produced), safe and robust inoculum at affordable costs remains a critical challenge. MycUpscaling addresses the challenging question of what are the genes responsible for increasing triacylglycerides (TAGs) accumulation in the symbiotic interface and increasing spore numbers to create a novel generation of high-quality and cost-effective AMF inoculants for application in agroecosystems.
The project will include combinatorial lipid metabolic engineering, selection of mycorrhized TAG-accumulating hosts, in vitro and in vivo lipid flux analysis, and in vitro spore domestication. We hypothesize that engineering lipid metabolism in mycorrhized plants will (i) increase TAG-based carbon sources in AMF, with spores accumulating more lipids for a higher root-colonization potential (bio-fortification=best quality), ii) stimulate the asexual reproduction machinery to produce more spores in plates and bioreactors (biomass production=high quantity), decreasing cost-fees of in vitro spore production systems (cost-efficiency=industry profitable).
MycUpscaling will employ an inter-disciplinary approach combining expertise of the researcher in cell engineering and his supervisors in plant lipid flux monitoring (WSU, USA) and large-scale AMF production (UCLouvain, Belgium). This project will enable the researcher to interact with key leading experts, re-inforce skills and competences, and forge a mature and outstanding international research carrer.
Fields of science
- engineering and technologyindustrial biotechnologymetabolic engineering
- agricultural sciencesagriculture, forestry, and fisheriesagricultureagronomyplant protection
- natural sciencesbiological sciencesmicrobiologymycology
- natural sciencesbiological sciencesbiochemistrybiomoleculeslipids
- natural sciencesbiological sciencesbiological behavioural sciencesethologybiological interactions
Programme(s)
Funding Scheme
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinator
1348 Louvain La Neuve
Belgium