Periodic Reporting for period 1 - CycloproBio (Cyclopropane Synthase Enzymes as Biocatalysts for Green Cyclopropanation Reactions)
Période du rapport: 2022-01-01 au 2023-12-31
Cycloprobio has characterised new CS enzymes and paved the way to their exploitation as biocatalysts for selective synthesis of cyclopropanated fatty acids and phospholipids. This ultimately is expected to contribute in reducing the environmental impact of a variety of chemical and pharmaceutical manufacturing processes. The project also firmly aligns with the key European research priorities of “green chemistry” and “sustainable manufacturing processes”.
Mutagenesis and in-silico experiments confirmed the role of key amino acids involved in the catalytic cyclopropanation and provided an insight into the lipid substrates preference of ecCFAS. Follow up work of semi-rational engineering of ecCFAS is currently ongoing to expand the substrate scope of the enzyme to new non-natural substrates. The results of the project is being disseminated to the scientific community via peer review open access publications and via specialized congresses contributions and will be shared with the broader public after publication, via social media.