Project description
Shedding light into centriole elimination during oogenesis
Centrioles are cellular organelles that support the formation of the locomotion structures cilia and flagella as well as centrosomes. During sexual reproduction, only the sperm contributes centrioles to the zygote, whereas centrioles are eliminated from the oocyte. Still, the underlying mechanism remains unknown. The EU-funded CENTEL project will employ Caenorhabditis elegans to unveil the mechanism by which centrioles are eliminated during oogenesis. Researchers will apply live imaging and conduct a proteomic screen to identify the key players in the process. Among the key objectives is to disclose the extent by which centriole elimination has been evolutionary conserved.
Objective
Centrioles are evolutionary conserved organelles that template the formation of cilia, flagella and centrosomes. Centriole number dictates how many such structures form in a cell, so that controlling this number is essential for proper development and health. During sexual reproduction of animals, only the sperm contributes centrioles to the zygote, whereas centrioles are eliminated from the oocyte. The signals initiating and the processes executing centriole elimination remain largely elusive, both during oogenesis and in other developmental contexts where this can occur. Here, I propose to conduct state-of-the art live imaging and use novel quantitative super-resolution approaches in C. elegans to uncover the timing and molecular changes during oogenesis centriole elimination. Moreover, I will decipher the role of the RNA-binding protein CGH-1 in the timing of centriole elimination through a sequencing-based approach. Furthermore, I will conduct a proteomic screen to identify and characterize novel components required for oogenesis centriole elimination in worms. In a complementary approach, I will address the potential evolutionary conservation of centriole elimination mechanisms using the protist N. gruberi, which diverged from C. elegans over a billion years ago. I propose to also analyze in detail the dynamics of the elimination process in this system using deployed molecular markers. Moreover, I will conduct a proteomic screen to identify candidate centriolar proteins directing centriole elimination in N. gruberi. Furthermore, I will develop RNAi-mediated and CRISPR/Cas9 inactivation methods to test the function of candidate proteins in centriole elimination, thus also adding an important item to the toolbox at the disposal of scientists using N. gruberi. Overall, my work is expected to reveal whether centriole elimination is an ancestral mechanisms that has been conserved across eukaryotes and uncover the underlying molecular tenets of this process.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences physical sciences optics microscopy super resolution microscopy
- natural sciences biological sciences biochemistry biomolecules proteins
You need to log in or register to use this function
We are sorry... an unexpected error occurred during execution.
You need to be authenticated. Your session might have expired.
Thank you for your feedback. You will soon receive an email to confirm the submission. If you have selected to be notified about the reporting status, you will also be contacted when the reporting status will change.
Keywords
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Project’s keywords as indicated by the project coordinator. Not to be confused with the EuroSciVoc taxonomy (Fields of science)
Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
-
H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
See all projects funded under this programme -
H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
See all projects funded under this programme
Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)
See all projects funded under this funding scheme
Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2020
See all projects funded under this callCoordinator
Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
1015 LAUSANNE
Switzerland
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.