Description du projet DEENESFRITPL Faire la lumière sur l’élimination des centrioles lors de l’ovogenèse Les centrioles sont des organites cellulaires qui soutiennent la formation des cils et des flagelles des structures de locomotion, ainsi que des centrosomes. Lors de la reproduction sexuée, seul le sperme apporte des centrioles au zygote, tandis que les centrioles sont éliminés de l’ovocyte. Toutefois, le mécanisme sous-jacent de cette élimination reste inconnu à ce jour. Le projet CENTEL, financé par l’UE, emploiera le ver Caenorhabditis elegans pour dévoiler le mécanisme par le biais duquel les centrioles sont éliminés lors de l’ovogenèse. Les chercheurs appliqueront des techniques d’imagerie en direct et réaliseront un criblage protéomique afin d’identifier les principaux acteurs du processus. Parmi ses principaux objectifs, l’équipe du projet souhaite déterminer l’étendue de conservation évolutive de l’élimination des centrioles. Afficher les objectifs du projet Masquer les objectifs du projet Objectif Centrioles are evolutionary conserved organelles that template the formation of cilia, flagella and centrosomes. Centriole number dictates how many such structures form in a cell, so that controlling this number is essential for proper development and health. During sexual reproduction of animals, only the sperm contributes centrioles to the zygote, whereas centrioles are eliminated from the oocyte. The signals initiating and the processes executing centriole elimination remain largely elusive, both during oogenesis and in other developmental contexts where this can occur. Here, I propose to conduct state-of-the art live imaging and use novel quantitative super-resolution approaches in C. elegans to uncover the timing and molecular changes during oogenesis centriole elimination. Moreover, I will decipher the role of the RNA-binding protein CGH-1 in the timing of centriole elimination through a sequencing-based approach. Furthermore, I will conduct a proteomic screen to identify and characterize novel components required for oogenesis centriole elimination in worms. In a complementary approach, I will address the potential evolutionary conservation of centriole elimination mechanisms using the protist N. gruberi, which diverged from C. elegans over a billion years ago. I propose to also analyze in detail the dynamics of the elimination process in this system using deployed molecular markers. Moreover, I will conduct a proteomic screen to identify candidate centriolar proteins directing centriole elimination in N. gruberi. Furthermore, I will develop RNAi-mediated and CRISPR/Cas9 inactivation methods to test the function of candidate proteins in centriole elimination, thus also adding an important item to the toolbox at the disposal of scientists using N. gruberi. Overall, my work is expected to reveal whether centriole elimination is an ancestral mechanisms that has been conserved across eukaryotes and uncover the underlying molecular tenets of this process. Champ scientifique sciences naturellessciences biologiquesbiochimiebiomoléculeprotéinesprotéomiquesciences naturellessciences physiquesoptiquemicroscopiemicroscopie à super-résolution Mots‑clés centrioles elimination organelle turn-over C. elegans oogenesis reproduction gamete formation Naegleria gruberi cellular differentiation Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Thème(s) MSCA-IF-2020 - Individual Fellowships Appel à propositions H2020-MSCA-IF-2020 Voir d’autres projets de cet appel Régime de financement MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinateur ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE Contribution nette de l'UE € 191 149,44 Adresse Batiment ce 3316 station 1 1015 Lausanne Suisse Voir sur la carte Région Schweiz/Suisse/Svizzera Région lémanique Vaud Type d’activité Higher or Secondary Education Establishments Liens Contacter l’organisation Opens in new window Site web Opens in new window Participation aux programmes de R&I de l'UE Opens in new window Réseau de collaboration HORIZON Opens in new window Autres sources de financement € 0,00
